木聚糖酶生产菌株的筛选及产酶条件的优化  

Screening and Condition Optimization of a Xylanase-producing Strain

作　　者：陈和秀[1] 龙敏南[2] 徐方成[1] 邬小兵[2] 徐惠娟[2] 刘清锋[2] 胡忠[2] 

机构地区：[1]厦门大学化学化工学院,厦门361005 [2]厦门大学生命科学院,厦门361005

出　　处：《中国生物工程杂志》2007年第11期41-44,共4页China Biotechnology

基　　金：国家"863"计划资助项目(2006AA05Z111);福建省重点科技平台资助项目(2006H0091)

摘　　要：以甘蔗渣半纤维素为碳源,从垃圾场土壤中分离到6株分解半纤维素的菌株。通过固态发酵的木聚糖酶活力比较,筛选到1株木聚糖酶活力较高的菌株。该菌株18SrDNA序列与曲霉(Aspergillussp.)的同源性达97%,根据对菌株形态学分析和18SrDNA序列分析的结果,将该菌株鉴定为曲霉HQ3。HQ3的最佳产酶条件为:甘蔗渣:麸皮为7:3(W/W),固液比为1:4(W/W),尿素0.4%,pH7.0,温度30℃,发酵产酶时间4d。在最佳产酶条件下,其木聚糖酶活最高可达3421U/g干曲。Six hemicellulose-decomposing strains were isolated from the refuse soil samples using bagasse hemicellulose as the sole carbon. The strain with high xylanase activity was screened and identified as Aspergillus sp. HQ3 by analysis of 18S rDNA sequences （97% similarity to Aspergillus sp. ） and phenotypic properties. The optimum solid-state fermentation conditions for xylanase production were obtained as ： bagasse ： bran = 7： 3 （ W/ W）, solid ： liquid = 1 ： 4 （ W/W）, urea 0.4 %, pH 7.0, fermentation temperature 30 ℃, fermentation time 4d. The activity of xylanase reached 3421 U/g koji under the optimum conditions.

关 键 词：曲霉 木聚糖酶 甘蔗渣 固态发酵 

分 类 号：Q813[生物学—生物工程]