天花粉蛋白对单纯疱疹病毒-1DNA复制的影响  

Inhibitive Effect of Trichosanthin on Herpes Simplex Virus-1DNA Replication in Vivo and Vitro

作　　者：陈光福[1] 尹飞[2] 张红媛[2] 黄文革[3] 陈凤英[3] 单金兰 

机构地区：[1]南方医科大学附属深圳医院儿科,广东深圳518035 [2]中南大学湘雅医院儿科,长沙410008 [3]中山大学动物实验中心,广州510000 [4]深圳市东湖医院检验科,广东深圳518000

出　　处：《实用儿科临床杂志》2007年第22期1693-1694,共2页Journal of Applied Clinical Pediatrics

基　　金：广东省医学科研基金项目资助(A2001629)

摘　　要：目的探讨天花粉蛋白对体内外单纯疱疹病毒-1(HSV-1)DNA复制的抑制作用。方法昆明小鼠40只随机分为模型对照组和天花粉蛋白治疗组,各20只。模型小鼠颅内接种HSV-1建立病毒性脑炎模型。治疗组于接种HSV-1前30min腹腔注射天花粉蛋白注射液;对照组予等量9g/L盐水。2组于接种病毒后12、24、48、96h分别测定脑组织HSV-1DNA复制水平。体外培养神经细胞分为病毒对照组和药物预处理组,接种病毒后12、24、48、96h分别测定各组培养神经细胞HSV-1DNA复制水平。结果天花粉蛋白治疗组于小鼠颅内接种HSV-1后12、24、48、96h脑组织HSV-1DNA复制水平分别为(1535±656、1468±608、1657±643、1425±534)×106拷贝/mL,显著低于模型组(4336±1350、5256±1837、5379±1975、5946±2769)×106拷贝/mL(Pa<0.001)。药物预处理组在12、24、48、96h后培养神经细胞HSV-1DNA复制水平分别为(1775±743、1347±687、1254±543、1276±525)×106拷贝/mL,显著低于病毒对照组(4653±1851,5120±2110,5045±1867,4995±2578)×106拷贝/mL(Pa<0.001)。结论天花粉蛋白对体内外HSV-1DNA复制具有抑制作用。Objective To explore the inhibitive effect of Trichosanthin（TCS） on herpes simplex virus - 1 （ HSV - 1 ） DNA replication in vivo and vitro. Methods Forty mice were randomly assigned into two groups： Model control group （ model group, n = 20 ）, TCS - treated group （treated group,n =20）. Viral encephalitis was induced by intracranial inoculation of HSV - 1 in the two groups. The TCS - treated group was injected with TCS 30 minutes before HSV - 1 inoculation. The control group was received equal dose 9 g/L saline. The HSV - 1 DNA replication were measured at 12,24,48,96 h after HSV - 1 inoculation, respectively. The culture neuron in vitro were assigned into two groups：HSV - 1 control group ,TCS -pretreated group. The HSV - 1 DNA replication were measured at 12,24,48,96 h after HSV - 1 inoculated into the neuron, respectively. Results The viral DNA replication of brain tissue at 12,24,48,96 h after HSV - 1 intracranial inoculation were markedly lower in the TCS - treated group （ 1 535± 656,1 468±608,1 657±643,1 425 ±534） × 10^6 copy/mL compared with the model group （4 336±1 350,5 256 ±1 837,5 379 ±1 975,5 946±2 769 ） × 10^6 eopy/mL,respectively （ Pa 〈 0. 001 ）. The HSV - 1 DNA replication of neuron at 12,24,48,96 h after HSV - 1 inoculation in TCS - pretreated group（ 1 775 ±743,1 347±687,1 254±543,1 276 ± 525 ） × 10^6 eopy/mL were markedly lower than those in the HSV - 1 control group（4 653± 1 851,5 120±2 110,5 045±1 867,4 995±2 578）× 10^6 copy/mL, respectively （Pa 〈 0. 001 ）. Conehlsion TCS has inhibitive effects on HSV - 1 DNA replication in vivo and vitro.

关 键 词：天花粉蛋白 疱疹病毒1型 人 DNA复制 脑组织 细胞培养 神经细胞 

分 类 号：R285.5[医药卫生—中药学]