nAChR介导茶多酚EGCG的神经保护作用  

Green tea catechins neuroprotection mediated by nAChR

作　　者：田建英[1] 杨浩 

机构地区：[1]宁夏医学院基础学院,宁夏银川750004 [2]第四军医大神经研究所,陕西西安710032

出　　处：《中国药理学通报》2007年第11期1494-1496,共3页Chinese Pharmacological Bulletin

基　　金：国家教育部优秀青年教师资助项目(No教人司2002[350]号);国家中药管理局资助项目(No04-05zp62)

摘　　要：目的探讨茶多酚EGCG(Epigallocatechin-3-gallate,表没食子儿茶素没食子酸酯)的神经保护作用机制。方法采用MTT比色分析,蛋白定量以及Dot Blot测定,观察Aβ1-40诱导缺损后,EGCG对α4、α7nAChR亚单位蛋白水平的改变。结果EGCG可抑制Aβ1-40诱导α4、α7nAChR亚单位蛋白水平下降和各类神经细胞活性的降低。结论茶多酚EGCG可通过上调α4、α7nAChR亚单位水平,抑制Aβ的神经毒性发挥神经保护作用,有可能成为防治记忆减退的有效途径。Aim To study whether or not the green tea catechins（EGCG）has physiological benefits and the underling protective mechanism.Methods The subunit protein levels of α4、α7 of nAChR were detected by BCA protein assay,Dot Blot assay and MTT assay.Results The results showed that the green tea catechins can significantly reduce the subunit protein levels of nAChR and decrease the cell activity induced by Aβ1-40.Conclusions EGCG can provide neuroprotection in vitro by up-regulating nAChR sununit levels and inhibiting the neurotoxin of Aβ1-40.

关 键 词：NACHR 茶多酚 AΒ 

分 类 号：R-332[医药卫生] R282.71