G-CSF对慢性粒细胞白血病患者bcr/abl^+-CD34^+细胞增殖、分化的影响  

Effect of G-CSF on the proliferation and differentiation of bcr/abl^+-CD34^+ cells from CML patients

作　　者：李澄宇[1] 孟凡义[2] 孙启鑫[2] 扶云碧[2] 江千里[2] 易正山[2] 宋兰林[2] 

机构地区：[1]广州医学院第二附属医院血液科,510260 [2]南方医科大学南方医院血液科,广州510515

出　　处：《中华血液学杂志》2007年第11期762-765,共4页Chinese Journal of Hematology

基　　金：广东省科技计划攻关课题(B30202)

摘　　要：目的了解 G-CSF 对 bcr/abl^+-CD34^+细胞增殖、分化的影响。方法采集慢性粒细胞白血病(CML)患者的 ber/abl^+-CD34^+细胞,分别以0、10、100、1000 ng/ml 的 G-CSF 与之共培养,并以正常骨髓 CD34^+细胞为对照,通过锥虫蓝拒染法、流式细胞术和光学显微镜观察研究其细胞增殖、周期分布、抗原分化和形态变化特点。结果所有实验组 ber/abl^+-CD34^+细胞均明显增长,其中 G-CSF10 ng/ml 组培养48、96 h,其细胞数量著高于同期无 G-CSF 组(P<0.05);而正常 CD34^+细胞数只在G-CSF 存在的情况下增长明显,其中 G-CSF 100 ng/ml 组培养48、96、144 h 细胞数均显著高于同期无G-CSF 组(P 值分别为<0.05,0.01,0.01);G-CSF 10、100、1000 ng/ml 组的 ber/abl^+-CD34^+细胞培养144 h 其 G_0/G_1期比例显著低于 G-CSF 空白组(P<0.05);而正常 CD34^+细胞 G-CSF 10、100、1000ng/ml 组培养48和96 h 其 G_0/G_1期比例均明显低于无 G-CSF 组(P<0.01);bcr/abl^+-CD34^+细胞及正常 CD34^+细胞 CD34抗原表达均随培养时间延长而下降,伴随 CD33和 CD13抗原先升后降,其变化与 C-CSF 浓度无关,但 ber/abl^+-CD34^+细胞的各抗原分化显著快于正常 CD34^+细胞。ber/abl^+-CD34^+细胞和正常 CD34^+细胞均随着增殖分化表现出终末细胞的形态特征。结论 G-CSF 能促进ber/abl^+-CD34^+细胞及正常 CD34^+细胞的增殖,但并非前者增殖的必要条件。ber/abl^+-CD34^+细胞比正常 CD34^+细胞分化更快,但两类细胞的分化速度均与 G-CSF 浓度无关。Objective To study the effect of granulocyte colony-stimulating factor （G-CSF） on the proliferation and differentiation of bcr/abl^＋-CD34^＋cells. Methods bcr/abl^＋-CD34^＋ cells were isolated from bone marrow of chronic myelocytic leukemia （CML） patients and were treated with 0, 10,100,1000 ng/ml of G-CSF for 48, 96, 144 hs. CD34^＋ cells from normal bone marrow were used as controls. Cell prolif- eration was determined by trypan blue dye exclusion, cell-cycle and antigen differentiation were determined by flow cytometry and cell morphology was observed under light microscope. Results The number of bcr/abl^＋-CD34^＋ cells was increased obviously in all groups. After cultured for 48 and 96 h, the number of bcr/abl^＋-CD34^＋ cells at G-CSF 10 ng/ml group was significantly higher than that in G-CSF 0 ng/ml group （P 〈 0.05） , the number of normal CD34^＋ cells was increased only in the presence of G-CSF. After cultured for 48, 96 and 144 h, the cell number in G-CSF 100 ng/ml group was significantly higher than that in G-CSF 0 ng/ml group （ P 〈 0. 05, P 〈 0. 01, P 〈 0.01, respectively）. After cultured for 144 h, the cell percentages in G0/G1 phase for bcr/abl^＋-CD34^＋ cells in G-CSF 10, 100, 1000 ng/ml groups were significantly less than that in G-CSF 0 ng/ml group （ P 〈 0.05）, and that for normal CD34^＋ cells in G-CSF 10, 100, 1000 ng/ml groups were significantly less than that of G-CSF 0 ng/ml group after cultured for 48 and 96 h. The expres- sions of CD34 on bcr/abl^＋-CD34^＋ cells and normal CD34^＋ cells were decreased along with the culture duration, accompanied by the expression of CD33 and CD13 increased first and decreased later, which was not correlated with the concentration of G-CSF. Both bcr/abl^＋-CD34^＋ cells and normal CD34^＋ cells showed ma- ture morphology along with proliferation and differentiation. Conclusions G-CSF promotes proliferation ofboth bcr/abl^＋-CD34^＋ cells and normal CD34^＋ cells, but not necessary for the former, and th

关 键 词：白血病 髓样 慢性 抗原 CD34 粒细胞集落刺激因子 细胞分化 

分 类 号：R733.7[医药卫生—肿瘤]