深山含笑叶片的挥发油成分及其生物活性研究  

Study on constituents and biological activity of volatile oil from leaves of Michelia maudiae

作　　者：操璟璟[1] 陈凤美[2] 龚玉霞[1] 李盼盼[2] 张小平[1] 

机构地区：[1]安徽师范大学国土资源与旅游学院,安徽芜湖241000 [2]徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《植物资源与环境学报》2007年第3期27-30,共4页Journal of Plant Resources and Environment

基　　金：安徽省自然科学基金资助项目(050430501);安徽省高等学校省级自然科学研究项目(KJ2007A092);2006年博士学科点专项科研基金(20060370001)

摘　　要：采用水蒸气蒸馏法及GC-MS联用技术提取并分析了深山含笑(Michelia maudiae Dunn)叶片的挥发油成分,并用杯碟法和Alamar blue法检测了其抑菌和抗肿瘤生物活性。从深山含笑挥发油中共分离出45种化合物,并鉴定出其中37种化合物,占挥发油总相对含量的97.8%;挥发油主要成分有莰烯(16.575%)、β-蒎烯(15.857%)、D-柠檬烯(12.653%)、丁香烯(11.923%)、1R-α-蒎烯(6.855%)及橙花叔醇(5.199%)等。该挥发油对金黄色葡萄球菌(Staphalococus aureus Rosenbach)和红酵母(Rhodotorula glutinisTrans.)有一定的抑制能力;对人非小细胞肺癌细胞(NCI-H460)有较强的抑制作用,浓度为100 mg.L-1,抑制率可达98.89%以上。The volatile oil was extracted from leaves of Michelia maudiae Dunn by steam distillation and its chemical components were analyzed by GC-MS technique.The results showed that 45 compounds were separated and 37 compounds were identified,which represented 97.8% of the total relative content of the volatile oil.The main constituents were camphene（16.575%）,βpinene（15.857%）,D-limonene（12.653%）,caryophyllene（11.923%）,1R-α-pinene（6.855%） and nerolidol（5.199%）.The biological activity of the volatile oil was screened with microbiological and Alamar blue methods.The experiment results indicated that the volatile oil possessed antibacterial activity to Staphalococus aureus Rosenbach and Rhodotorula glutinis Trans.,and it could also obviously inhibit the growth of cultured tumor cells（NCI-H460） with a inhibition rate reaching to 98.89% at volatile oil concentration of 100.00 mg·L^-1.

关 键 词：深山含笑 挥发油 气相色谱-质谱联用 生物活性 

分 类 号：S685.99[农业科学—观赏园艺] R284.2[农业科学—园艺学]