木聚糖酶的酶学性质研究  

Study on the Properties of Xylanase

作　　者：杨会涛[1] 陈代文[1] 田刚[1] 余冰[1] 

机构地区：[1]四川农业大学动物营养研究所四川省动物营养与饲料工程重点实验室,四川雅安625014

出　　处：《中国畜牧杂志》2007年第21期57-60,共4页Chinese Journal of Animal Science

基　　金：四川省科技攻关计划

摘　　要：采用DNS法对木聚糖酶的一些性质进行了研究。结果表明:该酶的最适反应pH为6.0,在pH5～10之间保温不同时间后可保持较高的活性;最适反应温度50℃,液态下低于60℃热稳定性较好。在50℃、pH6.0下,以燕麦木聚糖为底物时,该木聚糖酶的米氏常数Km=6.49mg/mL,最大反应速度Vmax=21.05μg/(mL·min)。用不同底物测定酶活,以燕麦木聚糖为底物的酶活最高。酶在动物胃中活性较低,而在肠道中活性较高。Properties of xylanases were studied by the method of DNS.The results showed that the optimal temperature and optimal pH of the xylanase was 50 ℃ and 6,respectively.Xylanase activity was stable between pH5 and pH10,and the themal stability of the enzyme was good in lipid below 60 ℃.The Km values and Vmax of xylanase for xylan from oat spelts under 50 ℃,pH6 were 6.49 mg/mL and 21.05 μg/（mL·min）,respectively.The xylanase had the highest activity when xylan from oat spelts was used as the substrate.The enzyme activity was inhibited in stomach and restored in small intestine.

关 键 词：DNS 酶学性质 稳定性 食糜 木聚糖酶 

分 类 号：Q814.9[生物学—生物工程]