小麦Wx-A1和Wx-B1亚基的生化与分子标记检测  

Biochemical and Molecular Detection of Wx-A1 and Wx-B1 Subunits in Wheat

作　　者：廉博[1] 郭世华[1] 侯国峰[1] 岳淑芳[1] 王秀娟[1] 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019

出　　处：《麦类作物学报》2007年第6期989-994,共6页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30460067);内蒙古自治区自然科学基金项目(200408020312)

摘　　要：为了解小麦品种(系)Wx亚基的缺失类型,为品质育种提供依据,利用SDS-PAGE和STS分子标记技术对99份国内外小麦品种(系)Wx-A1和Wx-B1亚基的缺失型进行检测。结果表明,供试小麦品种(系)中Wx-B1亚基缺失的有19份,未检测出Wx-A1亚基缺失材料。STS标记结果中,Wx-7A位点的显性标记引物在野生型中扩增出一条1 172 bp的特异带,即Wx-A1a基因出现;Wx-7A位点的共显性标记引物在野生型中扩增出一条593 bp的特异带,在缺失类型中扩增出一条574 bp的特异带。Wx-4A位点的显性标记引物在野生型中扩增出一条425 bp的特异带,即Wx-B1a亚基出现,而在缺失该亚基的突变材料中没有扩增出该特异带。For understanding the types of the waxy protein null cultivars and lines in wheat quality breeding,a total of 99 wheat cultivars were used in waxy protein null detection by SDS-PAGE and STS molecular marker.The results showed that there were 19 Wx-B1 null wheat cultivars and no Wx-A1 null cultivars.A 1172 bp fragment was detected for Wx-7A gene using dominant marker primer from the Wx-A1 normal wheat,a 593 bp and a 574 bp fragment were detected using codominant marker primer from the Wx-A1a normal and Wx-A1 null wheat,respectively.A 425 bp fragment was detected for using the Wx-4A dominant marker primer from the Wx-B1 normal wheats,whereas this fragment was absent in the Wx-B1a null wheats.

关 键 词：普通小麦 WX蛋白 SDS-PAGE 分子标记 

分 类 号：S152.1[农业科学—土壤学] S331[农业科学—农业基础科学]