亚麻籽木酚素的提取及其对裸鼠乳腺移植瘤生长的影响  

Isolation of flaxseed lignan and its effect on the growth of human breast cancer xenografts

作　　者：王晓蕾[1] 张莲英[1] 胡晓燕[1] 孙道旭[1] 吴伟芳[1] 刘传华[1] 刘贤锡[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,山东济南250012

出　　处：《中国生化药物杂志》2007年第3期176-178,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的分离、纯化亚麻籽木酚素——开环异落叶松树脂酚二葡萄糖苷(SDG),并研究其对裸鼠乳腺移植瘤生长的影响。方法从亚麻籽中提取、纯化SDG,高效液相色谱分析其纯度;选用人乳腺癌细胞株MDA-MB-231对裸鼠进行异种移植;第6周将移植成功的裸鼠随机分为3组:对照组、SDG组和亚麻籽组。每周测量瘤体大小并计算瘤表面积,同时记录裸鼠体重、摄食量。第10周处死动物,测量瘤体质量、瘤块大小,记录肿大淋巴结数。肝脏、肺脏进行病理切片检查。结果获得纯度为88.0%的SDG产品;与对照组相比,SDG、亚麻籽2组肿瘤表面积、瘤块质量、瘤体积明显降低(P<0.05),肿大淋巴结有所减少。各组动物体重及摄食量无明显差别,肝、肺均未见肿瘤转移。结论SDG可抑制裸鼠MDA-MB-231细胞乳腺移植瘤的生长。Purpose To isolate Secoisolariciresinol diglycoside（SDG） from flaxseed and to study its effect of on the growth of tumor in nude mice. Methods SDG was isolated from flaxseed and the purity was determined by high performance liquid chromatography （HPLC）. Nude mice were xenografted with human breast cancer cell line MDA-MB-231. At week 6,mice were randomly divided into 3 groups（n = 5 in each group） ： control group, SDG group and flaxseed group. Body weight and food intake were recorded weekly. Tumors were measured and the surface areas were calculated. Mice were sacrificed at week 10. At necropsy, body weight, primary tumor weight and volume and the number of intumescent lymph node were recorded. Lung and liver were analyzed pathologically. Results The tumor surface areas, tumor weight and volumes of the SDG group and flaxseed group were significantly lower than those of control group. The mean number of intumescent lymph node was also lowered in the two groups. No significant difference in food intake or body weight change was recorded between groups. No tumor metastasis in lung and liver was found in each group. Conclusion Dietary SDG can inhibit the growth of tumor in nude mice bearing human breast cancer cells MDA-MB-231.

关 键 词：亚麻籽 木酚素 开环异落叶松树脂酚二葡萄糖苷 乳腺癌 裸鼠 

分 类 号：R737.9[医药卫生—肿瘤] R285.5[医药卫生—临床医学]