分枝杆菌降解甾醇发酵生产雄烯二酮的两相系统培养基优化  

Medium Optimization of Androst-4-ene-3,17-dione Fermentation from Sterols by Mycobacterium sp. in Two-phase Systems

作　　者：姜绍通[1] 胡锦艳[1] 杨英[1] 赵俊平[1] 崔福强[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2007年第11期386-390,共5页Food Science

基　　金：国家"十一五"科技支撑计划项目(2006BAD05A12);安徽省自然科学基金项目(070413265X)

摘　　要：对分枝杆菌Mycobacterium sp.BD696-6降解甾醇发酵生产雄烯二酮(AD)的两相系统培养基进行了优化。在甾醇添加量为0.6%时,培养基组成为有机相葵花油浓度20%、碳源糖蜜6%、氮源NH4NO30.3%、磷酸盐(NH4)2HPO40.08%条件下,AD得率最高,达到78.6%,比原培养基条件下得率提高了17.0%。The two-phase system medium for Mycobacteriurn sp.BD696-6 to produce Androst-4-ene-3,17-dione（AD）by degrading sterols was optimized. The obtained optimal medium ingredients were： sunflower oil 20%, molasses 6%, NH4NO3 0.3%, （NH4）2HPO4 0.08%. Under the optimal medium determined, the conversion rate reached to 78.6% when the concentration of substrate steroid was 0.6%, which is17.0% higher than the original medium.

关 键 词：雄烯二酮 甾醇 两相系统 培养基优化 

分 类 号：Q939.97[生物学—微生物学]