采用cDNA芯片检测Krüppel样因子4过表达对C_2C_(12)细胞基因表达谱的影响  

Transcriptional Profiling of C_2C_(12) Cells Overexpressed Krüppel-Like Factor

作　　者：易宇欣[1] 刘瑛[1] 刘俊文[1] 张华莉[1] 周新民[2] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理学教研室 [2]湘雅二医院心胸外科,湖南省长沙市410078

出　　处：《中国动脉硬化杂志》2007年第8期585-590,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30400169;30571746;30571846;30330280);国家973重点资助项目(G2000056908)

摘　　要：目的采用cDNA芯片检测Krüppel样因子4过表达对C2C12细胞中基因表达谱的影响。方法用cDNA芯片检测Krüppel样因子4过表达的C2C12细胞中基因表达谱的改变(以空载体细胞为对照);用MEDLINE、Genomatix等生物信息学数据库和分析软件对在Krüppel样因子4过表达的C2C12细胞中表达发生改变的已命名基因进行功能和细胞信号通路分析。结果采用cDNA芯片筛选到在Krüppel样因子4过表达的C2C12细胞中表达发生改变的基因605个;其中下调的基因为250个,已命名基因为155个;上调的基因为355个,已命名基因为255个;其中包含多个炎症相关基因和凋亡相关基因。上述炎症相关基因属于13条细胞信号通路,凋亡相关基因属于9条细胞信号通路。结论Krüppel样因子4过表达能够引起C2C12细胞中多个下游基因表达发生改变。Aim To detect the change of transcriptional profiling of C2C12 cells overexpressed krüppel-like factor 4 （KLF4）. Methods eDNA chip containing 14000 mouse cDNAs were employed to investigate the expression of genes influenced by KLF4 overexpression in C2C12 cells; bioinformatics database and software such as MEDLINE and Genomatix were performed to analyze the above genes. Results Two hundred and fifity genes were down-regnlated in C2C12 cells overexpressed KLF4, with 155 known; Whereas 355 genes were up-restated, with 255 known. Several genes about inflammatory or apoptosis were included. Conclusion Many downstream genes were influenced by KLF4 ovcrexpression in C2C12 cells.

关 键 词：病理学与病理生理学 CDNA芯片 Krüppel样因子4 C2C12细胞 生物信息学 基因转染 信号通路 

分 类 号：R363[医药卫生—病理学]