大鼠局灶性脑缺血再灌注后早期生长反应基因—1mRNA的表达(英文)  

The expression of the early growth response gene-1 after focal cerebral ischemia / reperfusion in rats

作　　者：毕国荣[1] ■剑飞 白丽娟[1] 张贺敏[1] 张辉[1] 海虹[1] 周慧杰[1] 方秀斌[2] 

机构地区：[1]中国医科大学第二临床学院神经内科,沈阳110004 [2]中国医科大学基础医学院神经生物学教研室

出　　处：《中国神经精神疾病杂志》2007年第11期644-647,共4页Chinese Journal of Nervous and Mental Diseases

摘　　要：目的探讨早期生长反应基因-1( early growth response gene-1,Egr-1)mRNA在大鼠局灶性脑缺血再灌注后的表达情况。方法取10只健康雄性SD大鼠,体重200 ～250 g,应用原位杂交和RT-PCR方法检测大鼠局灶性脑缺血再灌注后Egr-1 mRNA的表达情况。结果 (1)原位杂交结果:假手术组神经元及胶质细胞为轻度阳性表达。缺血2 h再灌注2 h后,缺血侧Egr-1 mRNA细胞阳性表达明显增强。再灌注4 h时Egr-1 mRNA细胞阳性表达最高,再灌注22 h Egr-1 mRNA细胞阳性表达下降,至166 h时下降更加明显,但仍显著高于假手术组。(2)RT-PCR结果:大鼠脑缺血再灌注后缺血侧Egr-1 mRNA的表达明显高于假手术组,P<0.01。动态观察发现,脑缺血2h再灌注2 h后,Egr-1 mRNA即高表达,缺血2 h再灌注4 h达高峰,再灌注46 h已明显下降,但仍高于假手术组,P<0.01,随缺血再灌注时间延长,Egr-1 mRNA表达又逐渐增多,至再灌注166 h其表达亦明显高于假手术组,P<0.01。结论缺血再灌注后Egr-1 mRNA有规律性表达。Background To investigate the expression of the early growth response gene-I （ Egr-I） mRNA after focal cer- ebral ischemia / reperfusion in rats. Methods Ten healthy male SD rats weighing 200 -250 g were used to create model of focal cerebral ischemi- a. The expression of Egr-I after focal cerebral ischemia/reperfusion in rats was determined using in situ hybrid- ization and RT-PCR. Results （l） The result of the in situ hybridization： A trace amount of Egr-I mRNA expressed in the neurons and the glial cells in the sham operated group. The expression of Egr-I mRNA at the ischemic side increased dramatically following ischemia and reached peaks after 4 hours＇ reperfusion. Egr-I expression started to sub- side following 22 hours＇ reperfusion and further decreased following 166 hours＇ reperfusion, which was still significantly higher than that in the sham operated group. （2） The result of RT-PCR： The expression of Egr-I mRNA at the ischemic side was significantly higher than that in the sham operated group at all time points after ischemia/reperfusion in the rats（P 〈 0. 0l）. Expression of Egr-I increased 2 h after ischemia and reached the peak 4 h following reperfusion, and then decreased dramatically at 46 h after reperfusion which was still higher than that in the sham operated group （P 〈 0. 01）. As the ischemia/reperfusion period prolonged, the expres- sion of Egr-1 mRNA increased gradually, but still detectable even 166 h following reperfusion. The expression of Egr-1 was significantly higher than that in the sham operated group at all time points （P 〈0. 01）. Conclusions The expression of Egr-1 mRNA increase in the neurons and the glial cells after ischemia/reperfu- sion, which may have protective effects on ischemic brain tissues.

关 键 词：脑缺血再灌注早期生长反应基因-1 原位杂交RT-PCR 

分 类 号：R743.31[医药卫生—神经病学与精神病学]