产海藻糖合酶嗜热菌的筛选  

Screening of Thermophilic bacterium Producing Trehalose Synthase

作　　者：张峻[1] 陈晓云[1] 齐欣[1] 王成球 陈颖[1] 魏雪生[1] 

机构地区：[1]天津市园艺工程研究所,天津300384

出　　处：《食品与发酵工业》2007年第9期20-23,共4页Food and Fermentation Industries

基　　金：天津市自然科学基金(No.033605411);天津市重点新产品计划(No.043911711)资助项目

摘　　要：采用薄层层析及高效液相色谱对酶反应产物进行分析的方法,从地热水样中筛选出一株产胞内海藻糖合酶的嗜热菌CBS-01菌株。该菌株在固体培养基中细胞为杆状,在液体培养基中细胞通常为丝状,丝状体长度可变,好氧,革兰氏染色阴性,无芽孢,菌落呈圆形,表面光滑,凸起,边缘整齐,暗红色,生长温度范围为40～70℃,最适生长温度为55～60℃,生长pH范围为6.5～9.5,最适生长pH值为7.5～8.5,初步鉴定为亚栖热菌(Meiothermus sp.)。Thermophilic bacterium CBS-01, which can produce endocellular trehalose synthase, was isolated from geothermic water by analyzing the enzymatic reaction product with TLC and HPLC. The strain forms rod--shaped cells in solid medium or filaments with variable length in liquid medium. The cells are aerobic, Gram--negative and do not form endospores. Colonies are round, smooth, protruding, regular edged and dark red--pigmented. Growth occurs above 40℃ and below 70℃, the optimum growth temperature is about 55～60℃. The optimum pH is between 7.5 and 8. 5. Based on the above data, the organism was identified as a species of the genus Meiothermus.

关 键 词：海藻糖合酶 嗜热菌 筛选 

分 类 号：TQ920[轻工技术与工程—发酵工程]