姬松茸深层发酵生产菌丝体和胞外多糖的初步研究  

Preliminary Study on Producing Mycelium and Extracellular Polysaccharide in Submerged Fermentation of Agaricus blazei Murill

作　　者：刘高强[1] 王晓玲[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,湖南长沙410004

出　　处：《安徽农业科学》2007年第33期10707-10707,10722,共2页Journal of Anhui Agricultural Sciences

基　　金：湖南省教育厅资助科研项目(05C327)

摘　　要：[目的]研究姬松茸的深层发酵技术,筛选了适宜姬松茸液体深层培养的碳源和氮源培养基。[方法]对姬松茸的液体深层培养基进行了研究,筛选出适宜姬松茸发酵的最适碳源和氮源,并研究了不同浓度的最佳碳源和氮源对姬松茸菌丝体和胞外多糖的影响。[结果]姬松茸菌株生长和胞外多糖生产的最佳碳源为葡萄糖,最适浓度为30 g/L;最佳氮源为蛋白胨和酵母膏的组合,最适的组合浓度为7 g/L蛋白胨+7 g/L酵母膏。在此条件下,姬松茸菌体干重和胞外多糖产量均达到最大值,分别为8.7 g/L和281 mg/L。[结论]该研究为姬松茸的进一步规模化培养提供了参考。[Objective]The aim of the research was to study the submerged fermentation technique and screen out the medium with optimum carbon and nitrogen sources for submerged fermentation of Agaricus blazei Murill.[Method]Liquid submerged medium of A.blazei were studied,the optimum carbon and nitrogen sources for the fermentation of A.blazei were screened out and the effects of the optimum carbon and nitrogen sources with different concentrations on the mycelium and extracellular polysaccharide of A.blazei were studied.[Result]The optimum carbon source for the growth of strain and the production of extracellular polysaccharide of A.blazei was glucose and its optimum concentration was 30 g/L.The optimum nitrogen source was a combination of peptone and yeast extract and the optimum combination concentration was 7 g/L peptone ＋ 7 g/L yeast extract,under which the dry weight and extracellular polysaccharide of A.blazei thalli both reached maximum,being 8.7 g/L and 281 mg/L respectively.[Conclusion]The research provided references for further culture in large scale of A.blazei.

关 键 词：高等真菌 姬松茸 深层发酵 菌丝体 胞外多糖 碳源 氮源 

分 类 号：Q936[生物学—微生物学]