一株具有纤溶活性的枯草杆菌(Bacillus Subtilis)的研究——液体发酵条件的选择  

Study on a Protein Kinase Lysis Activity of Bacillus Subtilis HW-12 - Selection of liquid fermentation conditions

作　　者：傅俐[1] 李荣萍[1] 李晶[1] 张淑梅[1] 张云湖[1] 赵晓祥[1] 杨志兴[1] 

机构地区：[1]黑龙江省科学院应用微生物研究所

出　　处：《生物工程进展》1997年第3期31-33,共3页Progress in Biotechnology

摘　　要：本文主要报告了，以ＢａｃｉｌｕｓＳｕｂｔｉｌｉｓＨＷ－１２为试验菌株，在液体发酵中，所确定的发酵条件为：碳源是木糖，氮源是大豆胰蛋白胨，发酵液ｐＨ７．０，培养温度３７℃，培养时间９６小时。在这样的发酵条件下。In this paper we studied on ( Bacillus Subtilis ) HW-12 that yield high activity enzyme of protein Kinase.In liquid fermentation,we screened optimal fermentation conditions (medium,time,temperature et.al) for B.subtilis HW-12 in order to produce higer enzyme yield.Thus,it can provide scientific basis for enlarge culture.The optimal fermentation conditions were determined:carbon source:xylose;nitrogen source:tryptic soy broth;pH 7.0;temperature 37℃;96hr.In these conditions the stiain's enzyme activity is 200 urokinase unit each millilitre broth.

关 键 词：枯草杆菌 尿激酶 发酵 

分 类 号：TQ925.9[轻工技术与工程—发酵工程] TQ920.1