胃癌组织中Survivin外显子1甲基化状态的分析  

Aberrant demethylation of Survivin gene exon 1 in gastric cancer

作　　者：程正江[1] 胡丽华[1] 朱宇芳[2] 莫扬[2] 廖晓峰[3] 

机构地区：[1]华中科技大学同济医学院附属协和医院检验科,湖北武汉430022 [2]华中科技大学同济医学院附属襄樊医院检验科,湖北襄樊441021 [3]华中科技大学同济医学院附属襄樊医院普外科,湖北襄樊441021

出　　处：《中华肿瘤防治杂志》2007年第24期1885-1888,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究胃癌组织Survivin外显子1去甲基化与Survivin mRNA表达以及临床病理参数之间的关系。方法:25例胃癌及与其配对的正常胃组织DNA经甲基化敏感性限制性内切酶HpaⅡ和MspⅠ处理后用SYBR GreenⅠ实时荧光PCR对Survivin外显子1进行检测,Survivin mRNA的表达水平采用实时荧光定量PCR的方法进行检测。结果:Survivin外显子1去甲基化率在胃癌组织和癌旁组织分别为96.0%(24/25)和20.0%(5/25),χ2=29.639,P<0.001。在25例胃癌组织中有22例(88.0%)发生Survivin mRNA过表达,并且表达与去甲基化呈正相关,rs=0.5528,P=0.00416。没有发现去甲基化与年龄、性别、肿瘤大小、病理分级、临床分期和转移与否等病理参数之间存在统计学上的相关性,P>0.05。结论:Survivin外显子1去甲基化增强Survivin mRNA的表达,可能是胃癌发生和发展过程中的早期事件。OBJECTIVE：To analyze the correlation of aberrant demetbylation in the CpG site of Survivin gene exon 1 with Survivin mRNA expression and clinicopatbological characteristics of patients with gastric cancer. METHODS：The DNA samples from 25 pairs of gastric cancers and matched normal gastric tissues were digested with mCpG-sensitive Hpa Ⅱ and Msp Ⅰ , and detected by SYBR Green 1 real-time PCR with Survivin exon 1 specific primers. The expression of Survivin at the level of mRNA was determined by real-time quantitative RT-PCR. RESULTS：The demethylation rates of Survivin gene in cancer tissues and adjacent cancer tissues were 96.0%（24/25） and 20.0%（5/25）, respectively（χ^2 =29.639,P〈 0.001）. Among the 25 samples, there were 22（88.0%） samples with overexpressed Survivin mRNA, and the overexpression of Survivin mRNA was significantly associated with demethylation of Survivin exon 1 （r=0. 552 8,P=0. 004 16）. The aberrant demethylation pattern in CpG site of Survivin gene bad no close correlation with the clinicopathological characteristics （age, sex, tumor size, tumor grade, nodal status and histology type） of the patients with gastric cancer （P〉0. 05）. CONCLUSION： The aberrant demethylation in CpG site of Survivin gene exon 1 enhances Survivin gene expressing mRNA and may be regarded as an early molecular event in gastric cancer.

关 键 词：胃肿瘤/遗传学 外显子 甲基化 

分 类 号：R735.2[医药卫生—肿瘤]