猪生长肥育期背最长肌LPL基因表达与肌内脂肪含量相关研究  

Association of LPL gene expression with intramuscular fat contents in the longissimus dorsi muscle of pigs during growing-finishing period

作　　者：廉红霞[1] 卢德勋[2] 高民[2] 

机构地区：[1]内蒙古农业大学动科院 [2]内蒙古农牧业科学院动物营养所,内蒙古呼和浩特010030

出　　处：《畜牧与兽医》2007年第12期17-20,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家973重点基础研究发展计划(2004CB117500)

摘　　要：选取健康、体重接近10kg的杜洛克×长白×大约克去势公猪36头，体重达10，20，35，50，80，110kg时各屠宰6头。从背最长肌提取总RNA，反转录为cDNA，以猪B-actin基因作为内参，利用实时荧光定量RT-PCR技术，对各阶段LPL mRNA进行相对定量分析；结合IMF含量及大理石纹评分，分析LPL基因表达水平对IMF含量等的影响。结果表明：LPL mRNA、IMF含量及大理石纹评分随体重增加均呈上升趋势，其中LPL mRNA表达量80kg阶段显著高于10～50kg阶段（P〈0．05），肌内脂肪和大理石纹评分110kg均高于10～50kg阶段（P〈0．01）；IMF含量与大理石纹评分和LPL基因表达水平均呈现极显著正相关（P〈0．01）。Thirty-six healthy Duroc × Landrace × Yorkshire barrows （ 10 kg） were used, and 6 of them were exsanguinated when their body weights （BW） attained differently 10, 20, 35, 50, 80 and 110 kg in the current experiment. Genome RNA extracted from Longissimus Dorsi was reverse transcribed to cDNA. The TaqMan fluorescence quantitative reverse transcription-polymerase chain reaction （ FQ RT-PCR） was applied to determine the relative expression level of LPL mRNA in Longissimus Dorsi, and the β-actin gene was selected as internal control. The correlation between marble score or intramuscular fat （IMF） concentration and gene expression level of LPL mRNA was analyzed. The results showed that, marble score, IMF content and gene expression level of LPL mRNA were increased when BW increased during growingfinishing period. Gene expression level of LPL mRNA at 80 BW was significantly higher than that from 10 BW to 50 BW （P 〈 0. 05 ）, and IMF content and marble score at 110 BW were significantly higher than that from 10 BW to 50 BW （ P 〈 0. 01 ）. Moreover, the LPL gene expression level was positive correlated to IMF content and marble score significantly （P 〈 0. 01 ）.

关 键 词：猪 LPL基因 肌内脂肪 荧光定量RT-PCR 背最长肌 

分 类 号：S828[农业科学—畜牧学]