Eales病患者血液中氧化及抗氧化水平分析  

Analysis of oxidative and antioxidative levels in blood of patients with Eales disease

作　　者：肖骏[1] 魏世辉[2] 刘畅[3] 王洁[1] 苏冠方[1] 张晓光[1] 

机构地区：[1]吉林大学第二医院眼科,吉林长春130041 [2]解放军总医院眼科,北京100853 [3]吉林大学基础医学院生物化学与分子生物学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第6期1080-1083,共4页Journal of Jilin University：Medicine Edition

基　　金：解放军总后勤部十五面上项目资助课题(04M042);吴阶平医学基金会资助课题(2003-32-D);长春市科技计划资助课题(06GG162)

摘　　要：目的:通过对不同时期Eales病患者血液中氧化及抗氧化水平的比较分析,探讨氧化损伤与Eales病发病的关联性。方法:Eales病首发病例、治愈病例各10例为研究对象,同时选择健康男性献血队员10例为正常对照。以8-羟基脱氧鸟苷(8-OHdG)作为DNA氧化损伤的标记物,以巴比妥酸盐反应物质(TBARS)作为脂质过氧化的标记物,应用气相色谱质谱联用仪(GC/MS)及TBARS检测试剂盒测定不同时期Eales病患者血液中8-OHdG及丙二醛(MDA)的含量。应用相应的测定试剂盒分别对研究对象血清中总抗氧化能力、总SOD活力、GSH-PX活力及维生素E含量进行测定。结果:不同时期Eales病患者血液中8-OHdG及MDA含量均显著高于健康对照组(P<0.001);首发病例组血液中8-OHdG及MDA含量显著高于治愈病例组(P<0.001)。不同时期Eales病患者血清中总抗氧化能力、抗氧化酶活性及维生素E含量等抗氧化指标均较正常对照组显著下降(P<0.05)。另外,治愈病例组血清中抗氧化指标虽然较首发病例组有所升高,但仍显著低于正常对照组(P<0.05)。结论:氧化应激与Eales病的发病具有一定的关联性。推测补充维生素C、E等抗氧化物质可能对Eales病的治疗和预防起重要的作用。Objective To evaluate the oxidative and antioxidative levels in blood of patients with Eales disease in order to assess the relationship between the oxidant stress and Eales disease. Methods Twenty patients with Eales disease were enrolled in this study. Ten of them which were untreated before the study served as untreated group and the others which were clinical cured served as cured group. Ten healthy young blood donors were selected as control group. 8-OHdG and TBARS were served as biomarker of oxidative damage to DNA and lipid peroxidation, and were detected by GC/MS and TBARS assay kit respectively. The capacity of total antioxidant, SOD, GSH-PX and the level of vitamine E in the serum were served as the marker of the antioxidant capacity, and they were detected by the corresponding assay kits. Results Compared with control group, the levels of 8-OHdG and MDA increased significantly in both groups of the patients with Eales disease （P〈0. 001）. And the levels of 8-OHdG and MDA in untreated group was significantly higher than those in clinical cured group （P〈0. 001）. The antioxidantcapacity of the patients was significantly lower than that in control group （P〈0. 05）, and the antioxidant capacity in clinical cured group was significantly higher than that in untreated group （P〈0.05）. Conclusion The oxidant stress may play an important role in etiology and pathology of Eales disease. Antioxidant therapy might be beneficial to patients with Eales disease.

关 键 词：视网膜疾病 EALES病 氧化损伤 气相色谱质谱分析 抗氧化能力 

分 类 号：R774.1[医药卫生—眼科]