辐射调控性TK基因载体在胃癌细胞中的表达  

Expression of TK gene vecter regulated by ionizing radiation in gastric carcinoma cell line

作　　者：刘金龙[1] 刘训良[1] 郭仕英[2] 钱清[1] 杜青[1] 陈国玉[1] 

机构地区：[1]南京医科大学第一附属医院普通外科,210029 [2]南京师范大学生命科学学院分子医学实验室

出　　处：《江苏医药》2007年第12期1248-1250,共3页Jiangsu Medical Journal

摘　　要：目的利用放射敏感基因早期生长反应基因1(Egr-l)启动子驱动单纯疱疹病毒胸苷激酶基因(TK),观察其在胃癌细胞中的表达。方法以细菌内同源重组法构建含TK基因的腺病毒载体pAdEgr-1-TK,转染人胃癌细胞株MGC-803,分别以0、5、7.5、10、15、20Gy剂量γ射线照射,逆转录-聚合酶链反应(RT-PCR)半定量分析,比较TK基因的mRNA表达。结果转染后的胃癌细胞株经射线照射后其TK mRNA的表达较未照射组(56.4±1.46)%有明显提高,以10Gy最为显著(132.3±2.18)%,(P<0.01)。结论经γ射线照射后,射线激活Egr-l启动子可引起TK基因在胃癌细胞中高效表达,为进一步研究胃癌的放化疗提供了依据。Objective To observe the killing effect on gastric carcinoma（MGC-803） cell lines by early growth response-1 （Egr-1） promoter activated herpes simplex virus thymidine kinase（TK）. Methods Adenoviral vecter of pAdEgr-1-TK was generated through homologous recombination in bacteria and transfect human MGC-803. After exposure to 7-radiation by a ^60Co source, cellular TKmRNA expression in gastric carcinoma cells was compared by RT-PCR analysis. Results After irradiation, the TKmRNA expression of transfected cell lines was increased markedly compared with non-irradiation cell lines（ 56.4 ± 1.46）, especially in 10 Gy dosage（ 132.3 ± 2.18, P〈0.01）. Conclusion As a radio-inducible promoter, Egr-1 can activate TK expression significantly in transduced gastric carcinoma cell lines after irradiation. These in vitro data will provide an experimental basis for gene therapy and for further research of gene radiotherapy in MGC-803.

关 键 词：胃癌 Γ射线 生长反应基因1启动子 自杀基因 

分 类 号：R394[医药卫生—医学遗传学]