真菌产3种β-D-Glucuronidase酶学性质  

Characterization of three β-D-glucuronidase from Fungi

作　　者：王小艳[1] 冯世江[1] 文先军[1] 李晖[1] 李春[1] 

机构地区：[1]石河子大学绿色合成与转化实验室,石河子832003

出　　处：《生物加工过程》2007年第2期17-22,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20466002);新世纪优秀人才支持计划资助项目(NCET-04-0989);北京市自然科学基金资助项目(5072028)

摘　　要：分离筛选到能代谢甘草酸并产生不同产物的3株菌株,其中Penicillium sp.Li-3转化甘草酸生成GAMG,As-pergillus sp.Li-20生成GAMG和甘草次酸,Aspergillus sp.Li-62生成甘草次酸。对这3株菌表达β-D-葡萄糖醛酸苷酶的酶学性质进行了研究,结果表明：Penicillium sp.Li-3、Aspergillus sp.Li-20和Aspergillus sp.Li-62β-D-葡萄糖醛酸苷酶最适催化pH分别为4.2~4.6,5.8和6.2,最适催化温度为50、45和55℃。Penicillium sp.Li-3表达的β-D-葡萄糖醛酸苷酶Km为0.328μmol/L,Vmax为0.003 5 mmol/（L.min）;Aspergillus sp.Li-20β-D-葡萄糖醛酸苷酶Km为3.61 mmol/L,Vmax为0.034 mmol/（L.min）;而Aspergillus sp.Li-62β-D-葡萄糖醛酸苷酶Km为0.43 mmol/L,Vmax为0.106 mmol/（L.min）。The enzymatic properties and catalytic kinetics of three different β-D-glucuronidases from stonins the transformation of Glycyrrhizin were investigated. The optimal condition for Penicillium sp. Li-3 β-D-glucuronidase was pH 4.2 and temperature of 50 ℃ , the Km and maximum reaction rate was 0. 328 μmol/L and 0. 003 5 mmol/（ L· min） respectively, for the optimal condition. The optimal condition for Aspergillus sp. Li-20 β-D-glucuronidase was pH 5.8 and temperature of 45 ℃, the Km and maximum reaction rate was 3.61 mmol/L and 0. 034 mmol/（ L· min）, respectively, for the optimal conditions. The optimal condition for Aspergillus sp. Li-62 β-D-glucuronidase was pH 6.2 and temperature of 55 ℃, the Km and maximum reaction rate was 0. 43 mmol/L and 0. 106 mmol/（ L· min） respectively.

关 键 词：β-D-Glucuronidase 酶学性质 多样性 甘草酸 

分 类 号：TQ925[轻工技术与工程—发酵工程]