γ-生育三烯酚对人结肠癌HT-29细胞中DNA损伤作用的研究  

Study on γ-Tocotrienol induced DNA damage in human colon carcinoma HT-29 cells

作　　者：徐伟丽[1] 陈炳卿[1] 王琪[1] 刘慧坤[1] 孙向荣[1] 孙文广[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,黑龙江哈尔滨150081

出　　处：《疾病控制杂志》2007年第6期603-606,共4页Chinese Journal of Disease Control and Prevention

基　　金：国家自然科学基金(30471444)

摘　　要：目的探讨γ-生育三烯酚对结肠癌细胞中DNA的损伤作用。方法γ-生育三烯酚与结肠癌HT-29细胞共培养,采用单细胞电泳法检测γ-生育三烯酚对HT-29细胞中DNA的损伤程度。结果γ-生育三烯酚对人结肠癌细胞株HT-29细胞中DNA分子有较大程度的损伤,且与处理的浓度存在一定的相关性。结论γ-生育三烯酚对结肠癌细胞具有很强的细胞毒作用,在γ-生育三烯酚作用下HT-29细胞的DNA断裂损伤增加,可能就是γ-生育三烯酚抗肿瘤效应的重要作用机制之一。Objective To study the effect of γ-Tocotrienol on the DNA damage of colon cancer HT-29 cells. Methods The colon cancer HT-29 cells were cultured with γ-Tocotrienol in vitro. The DNA damage of colon cancer cells was determined by the single cell gel electrophoresis assay. Results γ-Tocotrienol could induce the DNA damage of colon cancer cell-lines HT-29 and show dose-response relationship. Conclusions The γ-Tocotrienol has great cytotoxic effect on colon cancer cells, the results indicating that the increasing of DNA damage in colon cancer may be a very important mechanism of γ-Tocotrienol anti-tumor effects.

关 键 词：生育三烯酚类 结肠肿瘤 DNA损伤 

分 类 号：R735.35[医药卫生—肿瘤]