糖对溶胶-凝胶法固定化脂肪酶CALB的影响  

Effect of sugar on lipase CALB entrapped in sol-gel glass matrix

作　　者：高静[1] 檀文礼[1] 黄志红[1] 李伟杰[1] 贺莹[1] 

机构地区：[1]河北工业大学化工学院,天津300130

出　　处：《化工学报》2007年第12期3077-3081,共5页CIESC Journal

基　　金：国家自然科学基金重点项目(20536040);河北省教育厅自然科学研究项目(2002150)~~

摘　　要：Sugar was selected for protecting Candida antarctic lipase B entrapped in sol-gel glass matrix.The activities of immobilized CALB influenced by sucrose,glucose,trehalose,xylose and maltose were investigated.The result showed that trehalose,glucose and xylose were the best protector for lipase CALB.By means of infrared spectrometry and differential scanning calorimeter,the protection mechanism of immobilized CALB by xylose was studied.The results showed that xylose bonded with lipase provided significant improvement in the stability of entrapped CALB.Sugar was selected for protecting Candida antarctic lipase B entrapped in sol-gel glass matrix.The activities of immobilized CALB influenced by sucrose,glucose,trehalose,xylose and maltose were investigated.The result showed that trehalose,glucose and xylose were the best protector for lipase CALB.By means of infrared spectrometry and differential scanning calorimeter,the protection mechanism of immobilized CALB by xylose was studied.The results showed that xylose bonded with lipase provided significant improvement in the stability of entrapped CALB.

关 键 词：溶胶-凝胶 脂肪酶 固定化 乳酸乙酯 

分 类 号：O647[理学—物理化学]