仙人掌多糖对蛋白质糖基化终产物和醛糖还原酶的抑制效应(英文)  

Inhibition of Polysaccharides from Opuntia monacantha on Development of Advanced Glycated End Products and Activity of Aldose Reductase

作　　者：杨宁[1] 赵谋明[1] 杨宝[2] 朱邦豪[3] 方芳[3] 

机构地区：[1]华南理工大学轻工与食品学院,广州510640 [2]中国科学院华南植物园,广州510650 [3]中山大学基础医学院,广州510080

出　　处：《天然产物研究与开发》2007年第4期568-571,共4页Natural Product Research and Development

基　　金：The project was supported by Guangdong Provincial Scientific Research Foundation(No.2005B20401024);Guangzhou Scientific Research Foundation(No.2004Z1-E0061).

摘　　要：以仙人掌为原料提取多糖产品,通过超滤浓缩和不同浓度乙醇分级沉淀,分别得到55%乙醇沉淀的多糖(OPMC I),进一步乙醇浓度增至80%沉淀的多糖(OPMCⅡ),以及一次性80%乙醇沉淀的多糖(OPMCⅢ)。比较了三种多糖对蛋白质非酶糖基化反应的终端产物(AGEs)和醛糖还原酶(AR)形成的抑制作用。结果表明,在对AGEs形成的抑制过程中,第四周时OPMC II的抑制作用较强,且强于同剂量的氨基胍;在对AR活性的抑制作用中,OPMC II在三种多糖样品中抑制作用最强,但明显低于阳性对照物依帕司他。Polysaccharides from Opuntia monacantha Cladode （OPMC） were extracted by distilled water and concentrated by ultrafiltration. OPMC Ⅰ was obtained by precipitation OPMC extracts with 55% of ethanol solution. The supernatant was added ethanol to final concentration 80% to precipitate OPMC Ⅱ. OPMC Ⅲ was obtained by precipitation OPMC extracts with 80% of ethanol solution directly. The inhibition of three OPMC polysaccharides on the development of advanced glycated end product （AGE） of protein non-enzymaticaly glycated reaction and aldose reductase （AR） activity were investigated. OPMC Ⅱ showed stronger inhibition effect on AGE than aminoguanidine with the same dose at fourth week. Among OPMC Ⅰ -Ⅲ ,OPMC Ⅱ also showed the strongest inhibition effect on AR activity, but lower than epalrestat ,the positive control.

关 键 词：仙人掌 多糖 糖基化终产物 醛糖还原酶 抑制 

分 类 号：Q946.91[生物学—植物学] R282.71[医药卫生—中药学]