甲状腺乳头状癌组织中RASSF1A基因启动子甲基化与临床病理学的关系  

Study on Promoter Methylation of RASSF1A Gene in Papillary Thyroid Carcinoma

作　　者：唐建东[1] 欧阳军[2] 栗夏莲[1] 杜潇潇[1] 邱新光[3] 张全武[4] 

机构地区：[1]郑州大学第一附属医院内分泌科,450052 [2]河南省医学科学研究所 [3]郑州大学第一附属医院甲状腺外科 [4]郑州市中心医院病理科

出　　处：《天津医药》2007年第12期887-889,共3页Tianjin Medical Journal

摘　　要：目的:探讨甲状腺乳头状癌及癌旁组织中RASSF1A基因启动子甲基化改变的特点与临床特征的关系。方法:从甲状腺乳头状癌及癌旁组织中提取基因组DNA,采用甲基化特异性PCR检测RASSF1A基因启动子甲基化情况。结果:(1)甲状腺乳头状癌组织中RASSF1A基因启动子甲基化的发生率为55.9%(19/34),癌旁组织中RASSF1A基因启动子的甲基化的发生率为23.5%(8/34),癌组织中RASSF1A基因启动子甲基化显著高于癌旁组织(P<0.05)。(2)有淋巴结转移的甲状腺乳头状癌组织RASSF1A基因启动子的甲基化的发生率为77.8%(14/18),高于无淋巴结转移组的31.3%(5/16),差异有统计学意义(P<0.05)。(3)临床Ⅱ～Ⅲ期甲状腺乳头状癌患者中RASSF1A基因启动子的甲基化发生率为88.9%(8/9),高于Ⅰ期的44.0%(11/25),差异有统计学意义(P<0.05)。结论:甲状腺乳头状癌组织中存在RASSF1A基因启动子区的异常甲基化,甲基化可能与甲状腺乳头状癌发生发展过程有关。Objective： To investigate the relationship between the characteristics of promoter methylation of RASSF1A gene in papillary thyroid carcinoma （PTC） and the clinical manifestation of PTC. Methods： In 34 patients with PTC ,the thyroid genomic DNA of tumor tissues and adjacent tissues were purified by standard methods. The methylation status of RASSF1A gene was detected by methylation-specific PCR technique （MSP）. Results： The methylation rate of RASSF1A gene in PTC tissues was 55.9% （ 19/34 ）, while the methylation rate of RASSF 1A gene in adjacent thyroid tissues was 23.5% （8/34）. The rate of methylation of RASSF1A promoter in PTC carcinoma was significantly higher than that of adjacent thyroid tissues （P 〈 0.05）. The rate of methylation in PTC patients with lymph nodes metastasis was 77.8% （14/18）, significantly higher than that without lymph nodes metastasis 31.3% （5/16 ,P 〈 0.05 ）. The rate of methylation of RASSF1A gene inPTC of stageⅡ-Ⅲ was 88.8% （8/9）,significantly higher than that in PTC of stage I （44.0%,11/25,P〈 0.05）. Conclusion： Abnormal promoter methylation of RASSF1A in PTC may be involved in the development and progression of PTC.

关 键 词：甲状腺肿瘤癌 乳头状 基因 RAS 启动区(遗传学) 甲基化 聚合酶链反应 

分 类 号：R737.14[医药卫生—肿瘤]