猪心苹果酸脱氢酶的分离纯化及性质  

Preparation and Characterization of Malate Dehydrogenase from Swine Heart

作　　者：张大伟[1] 杨海麟[1] 王武[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2007年第6期90-94,共5页Journal of Food Science and Biotechnology

摘　　要：采用组织破碎,二度硫酸铵盐析,DEAE Sepharose F.F.离子交换层析及Sephadex G—75 Fine凝胶过滤等纯化方法,从猪心中提取得到了苹果酸脱氢酶。提取纯化的苹果酸脱氢酶经SDS—PAGE测定显示为单一条带,相对分子质量为34000。酶活回收率为57.99%,最适pH为8.0,最适温度为50℃。By tissue crushing, ammonium sulfate duel-precipitation and purification through DEAE Sepharose F. F. Sephadex G-75 filtration, the malate dehydrogenase （MDH） from swine heart was prepared. The purified MDH was illustrated as a single band in SDS--PAGE. molecular weight of MDH subunit was 34 000. The recovery of MDH activity was 57.99%. optimum pH for MDH was 8.0, and the optimum temperature for MDH was 50℃. The The

关 键 词：猪心 苹果酸脱氢酶 柱层析 酶学性质 

分 类 号：Q814.1[生物学—生物工程]