胶束增敏荧光法同时测定牛乳中残留的依诺沙星和左氧沙星  

Simultaneous Determination of Enoxacin and Levofloxacin Residue in Milk by Fluorescent Spectrophotometry Sensitized with Micelle

作　　者：路杨[1] 陈冠华[1] 

机构地区：[1]河北农业大学生命科学学院

出　　处：《中国食品学报》2007年第6期124-128,共5页Journal of Chinese Institute Of Food Science and Technology

摘　　要：在pH3.65的柠檬酸盐缓冲溶液中,当十二烷基硫酸钠胶束的浓度为80mmol/L时,依诺沙星和左氧沙星可发出较强荧光。通过对激发波长和发射波长的适当选择,可对二者进行同时测定。在最佳条件下,依诺沙星和左氧沙星的检出限分别为2.90μg/L和2.11μg/L,线性动态范围分别为50～600μg/L和25～350μg/L。该方法已用于乳中依诺沙星和左氧沙星的残留测定,二者的加标回收率分别在101.29%～113.67%和85.63%～91.93%之间。Enoxacin and levofloxacin can emit intense fluorescence in the citrate buffer adjusted to pH 3.65 when the SDS concentration in the buffer is 80 mmol/L. The two analytes can be simultaneously determined by means of the proper selection for exciting wavelength and emitting wavelength. Under the optimum conditions, the detection limits are 2.90 μg/L for enoxacin and 2.11 μg/L for levofloxacin, and the linear dynamic ranges are 50-600 μg/L for enoxacin and 25-350 μg/L for levofloxaein, respectively. The method has been applied to the determination of enoxacin and levofloxacin residue in milk, and the recoveries of spiked sample are 101.29%-113.67% for enoxacin and 85.63%-91.93% for levofloxacin.

关 键 词：依诺沙星 左氧沙星 荧光分光光度法 十二烷基硫酸钠 牛乳 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]