PTEN基因甲基化及其表达异常与胃癌的关系  

Promoter Methylation and mRNA Expression of the PTEN Gene in Gastric Carcinoma

作　　者：刘嵩[1] 于皆平[2] 付萍 于红刚[2] 

机构地区：[1]武汉市第一医院消化科,武汉市430022 [2]武汉大学人民医院消化科 [3]武汉市第十四医院内三科

出　　处：《中国肿瘤临床》2007年第24期1383-1386,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:30300154)

摘　　要：目的:探讨PTEN基因表达、启动子区甲基化与胃癌及其临床病理特征的关系。方法:采用甲基化特异性PCR法(MSP)和逆转录聚合酶链反应(RT-PCR)法,分析胃癌组织及相应癌旁正常组织中PTEN基因启动子区甲基化及其mRNA表达情况。结果:48.2%(27/56)的胃癌组织和3.6%(2/56)的癌旁胃组织PTEN基因启动子区发生甲基化,癌组织PTEN基因启动子区甲基化率显著增高(P<0.05)。其中,2例甲基化癌旁胃组织均为胃癌组织中有甲基化的病例。发生淋巴结转移的29例胃癌组织中,19例PTEN基因启动子甲基化;发生淋巴结转移的PTEN基因启动子甲基化显著高于无淋巴结转移组(P<0.05)。RT-PCR结果显示,所有甲基化的胃癌组织中PTEN mRNA均无表达。结论:胃癌中PTEN基因mRNA失表达与其启动子区甲基化有关,这可能是胃癌发生、发展以及转移的原因之一。Objective： To detect promoter methylation and mRNA expression of phosphatase and tensin homologue （PTEN） in gastric carcinoma and to analyze their relationship with the clinical and pathological features. Methods： Methylation-specific PCR and RT-PCR were employed to detect promoter methylation and mRNA expression of the PTEN gene in gastric cancer tissues and their adjacent tissues from 56 patients. Results： Promoter methylation was found in 48.2% （27/56） of the carcinoma tissues and 3.6% （2/56） of carcinoma-adjacent tissues, and the difference was significant （P〈0.05）. In the 29 cases with lymph node metastasis, 19 cases were observed with PTEN promoter methylation. PTEN mRNA was not expressed in tissues with methylation of the PTEN promoter. Conclusion： Loss of PTEN transcription is associated with methylation of its an important role in the carcinogenesis and metastasis of promoter in gastric carcinoma and it may play gastric carcinoma.

关 键 词：PTEN基因 甲基化 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]