一株内生真菌单独及与水稻联合降解菲的研究  

The degradation of phenanthrene by endophytic fungi Phomopsis sp. single and co-cultured with rice.

作　　者：田林双[1] 戴传超[1] 赵玉婷[1] 赵沫[1] 勇应辉[1] 王兴祥[2] 

机构地区：[1]南京师范大学生命科学学院江苏省生物多样性与生物技术重点实验室,江苏南京210046 [2]中国科学院南京土壤研究所,江苏南京210008

出　　处：《中国环境科学》2007年第6期757-762,共6页China Environmental Science

基　　金：国家"973"项目(2005CB121107);国家自然科学基金资助项目(30500066)

摘　　要：对一株植物内生真菌拟茎点霉(编号B3)单独降解菲的条件及与水稻联合降解菲进行了研究.结果表明,乳糖和蛋白胨浓度均为10g/L、愈创木酚作为诱导物时,B3菌株10d内对初始浓度为100mg/L菲降解率达52.54%.该菌对菲的降解与菌丝生物量、胞外木质素降解酶类无明显关系,其分泌的漆酶作用底物表现出一定的特殊性.B3菌株与其共生植物水稻联合培养有利于对菲的降解,30d后菲残留浓度仅为无菌对照组的8.40%.水稻感染内生真菌B3后对菲胁迫表现出一定的缓解作用.The degradation of phenanthrene by Phomopsis sp. （strains No. B3） single and co-cultured with rice were studied. When the concentration of lactose and peptone were 10g/L respectively, guaiacol acted as inducer, the degradation rate ofphenanthrene （100mg/L） reached 52.54% after 10 days rotary culture. No apparent correlation between degradation efficiency and the tested ligninolytic enzymes and the biomass. When B3 was co-cultured with paddy rice, the degradation rate of phenanthrene was enhancement, the concentration of phenanthrene was only 8.40% of that of the control after 30 days. In addition, B3 decreased the injury to rice under the condition of phenanthrene stress.

关 键 词：内生真菌 拟茎点霉 菲 降解 漆酶 胁迫 

分 类 号：X172[环境科学与工程—环境科学]