纤溶酶高产菌株筛选及其液体发酵条件研究  

Study on Strains Selection and Liquid Fermentation Conditions of Fibrinolytic Enzyme

作　　者：周伏忠[1] 贾蕴莉[1] 陈国参[1] 谢宝恩[1] 王红云[2] 

机构地区：[1]河南省微生物工程重点实验室,郑州450008 [2]河南省科学院生物研究所有限责任公司,郑州450008

出　　处：《河南科学》2008年第1期45-49,共5页Henan Science

基　　金：河南省省属科研单位社会公益项目(0641180203)

摘　　要：研究实验室自主分离的2株纤溶酶高产菌株发酵条件,采用4因素、3水平的正交试验设计方法对液体发酵培养基的碳源、氮源、无机盐进行了优化,并对培养基初始pH、发酵时间、发酵温度对产酶量的影响进行了测定.结果表明:菌株1液体发酵合适的产酶培养基配方为大豆粉的质量分数为6%,葡萄糖2%,CaCl2 0.06%,MgSO4 0.07%;菌株2液体发酵合适的产酶培养基配方为大豆粉的质量分数为6%,玉米淀粉2%,CaCl2 0.06%,MgSO4 0.07%.培养基初始pH均为6.5时酶活性最高;发酵温度均以38℃最好;而菌株1和菌株2的适宜发酵时间分别为48～84 h和60～84 h.在优化条件下,菌株1、菌株2液体发酵最高酶活分别为306.46 IU/mL和319.76 IU/mL。In this study, we separated two excellent fibrinolytic enzyme producing strains from the traditional soybean diets; Then we tested the factors influencing the fibrinolytic enzyme activity at the liquid culture, these factors include carbon source, nitrogen source inorganic salts, medium pH,cuhure temperature and times. The results show that about strain 1, the suitable medium were consist of 6 % soybean powder, 2 % dextrose, 0.06 % CaC12, 0.07 % MgS04 pH 6.5; about strain 2, the suitable medium were consist of 6 % soybean powder, 2 % corn starch powder, 0.06 % CaCI2, 0.07 % MgS04 pH 6.5. The best culture temperature for the two strains were all 38℃; and the suitable culture time of them were 48-84 h （strain 1） and 60-84 h （strain 2） respectively. At the suited fermentation conditions, the flask was 306.46 IU/mL and strain 2 was 319.76 IU/mL highest fibrinolytic enzyme activity of strain 1 in liquid respectively.

关 键 词：纤溶酶 菌株筛选 液体发酵 

分 类 号：Q939.9[生物学—微生物学]