山楂叶中总黄酮的含量测定方法研究  

Determination of Total Flavonoids in Leaves of Crataegus L.

作　　者：刘荣华[1] 邵峰[1] 马志林[1] 余伯阳[2] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004 [2]中国药科大学中药复方研究室,南京210038

出　　处：《江西中医学院学报》2007年第6期37-40,共4页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30472161);江西省自然科学基金资助项目(0640163)

摘　　要：目的:研究山楂(山里红)叶中总黄酮的含量测定方法。方法:选择山里红叶中某一特定化学成分(牡荆素2″-O-鼠李糖苷),比较该成分与总黄酮的含量关系,计算其比值,以该比值为换算因子,通过测定该成分的含量推算出山里红叶中总黄酮的含量。结果:不同产地、不同采收期16个山里红叶样品中牡荆素2″-O-鼠李糖苷与总黄酮的含量成显著正相关(r=0.950,P<0.01),且总黄酮与牡荆素2″-O-鼠李糖苷的含量比值(换算因子)为1.9493±0.2002。结论:可以将牡荆素2″-O-鼠李糖苷的含量乘以换算因子求算出不同产地、不同采收期山里红叶中总黄酮的含量。Objective：Studying the method of determination of total flavonoids in leaves of Crataegus pinnatifida Bge.Var.major N.E.Br.Methods：Vitexin 2″-O-rhamnoside was selected as the standard.The ratio of Vitexin 2″-O-rhamnoside and the total flavonoids in leaves of Crataegus pinnatifida Bge.Var.major N.E.Br.was calculated.The content of total flavonoids in leaves of Crataegus pinnatifida Bge.Var.major N.E.Br.could be counted with the result of the content of the standard multiplied by the ratio.Results：The correlation of the content of Vitexin 2″-O-rhamnoside and the total flavonoids in 16 kinds of leaves of Crataegus pinnatifida Bge.Var.major N.E.Br.from different locations and growth stages is significant,and the correlation coefficient was 0.950（P〈0.01,2-tailed）.The ratio of Vitexin 2″-O-rhamnoside and the total flavonoids in leaves of Crataegus pinnatifida Bge.Var.major N.E.Br.was 1.9593±0.2002.Conclusion：The total flavonoids in leaves of Crataegus pinnatifida Bge.Var.major N.E.Br.from different locations and growth stages could be calculated with the result of the content of Vitexin 2″-O-rhamnoside multiplied by the ratio.

关 键 词：山楂 山里红 黄酮 药物分析 

分 类 号：R284.1[医药卫生—中药学]