共底物混菌发酵产1,3-丙二醇研究  

Study on Co-culture with Co-substrate for the Production of 1,3-propanediol

作　　者：陈国[1] 赵亚囡[2] 姚善泾[2] 张光亚[1] 方柏山[1] 

机构地区：[1]工业生物技术福建省高等学校重点实验室华侨大学,福建泉州362021 [2]浙江大学化学工程与生物工程系,浙江杭州310027

出　　处：《四川大学学报（工程科学版）》2007年第5期64-69,共6页Journal of Sichuan University (Engineering Science Edition)

基　　金：国家自然科学基金资助项目(20676048);福建省青年人才资助项目(2006F3080);华侨大学启动基金资助项目(06BS214)

摘　　要：以葡萄糖和甘油作为共底物培养基,将利用葡萄糖产甘油的酵母Candida krusei和利用甘油产1,3-丙二醇的细菌Klebsiella pneumoniae进行混菌培养,考查了双菌混合培养转化葡萄糖生成1,3-丙二醇的能力。首先考察了不同初始甘油和葡萄糖浓度对发酵结果的影响,在葡萄糖浓度为100 g/L和甘油浓度为80 g/L时得到较好的结果。然后在混合培养基中,对Candida krusei和Klebsiella pneumoniae分别独立培养时的生长情况,与双菌混合培养时的菌体生长情况作了比较,并同时对底物的利用和产物的生成情况作了比较。结果表明,葡萄糖的加入会抑制Klebsiella pneumoniae的生长,但是随着Candida krusei对葡萄糖的不断利用,葡萄糖对Klebsiella pneumoniae的生长的抑制会减弱。双菌混合培养可以将部分葡萄糖转化为1,3-丙二醇,但是其转化效率不高。Candida krusei which consumed glucose to produce glycerol and Klebsiella pneumoniae which consumed glycerol to produce 1,3 - propanediol were co-cultured in a co-substrate medium composed of glucose and glycerol to study their ability of convering glucose to 1,3 - propanediol. The effect of initial glucose and glycerol concentration on products concentration was investigated firstly. The results showed that higher 1,3 - PD concentration was obtained at the initial glucose concentration of 100 g/L and initial glycerol concentration of 80 g/L. The growth curves of individual Candida krusei, Klebsiella pneumoniae and co-culture in co-substrate medium were compared, and correspondingly the glucose, glycerol, 1,3 -PD concentration curves were compared. The results showed that the present of glucose in medium would inhibit the growth of Klebsiella pneumoniae, especially in individual culture in co-substrate medium, but with the consumption of glucose by Candida krusei the inhibition function was decreasing. Co-cuhure of Candida krusei and Klebsiella pneumoniae converted partial glucose into 1,3 - PD, but the yield was lower and the correlation of Candida krusei and Klebsiella pneumoniae deserved advance research.

关 键 词：混菌培养 发酵 甘油 1 3-丙二醇 

分 类 号：TS201.2[轻工技术与工程—食品科学]