IGF-1对缺血性脑损伤大鼠脑内神经发生的影响  

Effects of IGF-1 on Neurogenesis after Focal Cerebral Ischemia in Rats

作　　者：王琳娜[1] 崔景彬[2] 

机构地区：[1]中南大学湘雅医院,湖南长沙410078 [2]郑州大学医学院,河南郑州450052

出　　处：《现代生物医学进展》2008年第1期30-33,共4页Progress in Modern Biomedicine

基　　金：河南省科技攻关项目(324410034)

摘　　要：目的:建立大鼠单侧局灶脑缺血模型,观察胰岛素样生长因子-1(IGF-1)对局灶脑缺血后的鼠脑神经发生及增殖后细胞生存的影响。方法:用健康雄性SD大鼠建立大脑中动脉阻塞(MCAO)模型,随机分成假手术组,缺血对照组和IGF-1治疗组。各组再按不同的治疗时间分为7d、14d、28d、42d组。免疫组化法观察BrdU、PSA-NCAM的变化,免疫双标法观察BrdU/PSA-NCAM、BrdU/MAP_2和BrdU/GFAP的共同表达变化。结果:BrdU标记细胞和PSA-NCAM标记细胞计数均在缺血后第7d最多,分别是缺血对照组的4.0倍和1.8倍。是假手术组的9.9倍和5.4倍。BrdU和PSA-NCAM双标细胞在缺血发生后双侧SVZ和DG区可以检测到,于第7d计数最多,之后逐渐降低;而BrdU和MAP_2以及BrdU和GFAP双标细胞却从第14d开始逐渐增多,直到第42d。随着BrdU/PSA-NCAM双标阳性表达的逐渐降低,BrdU/MAP_2双标阳性表达逐渐增高,呈现此消彼涨的变化。结论:IGF-1侧脑室注射后,在早期(7d内)诱导了缺血性脑损伤后神经细胞的增殖;在中期(7d-14d)诱导了新生细胞的迁移;在后期(14d后)伴随着迁移的进行新生细胞逐渐发生了分化。Objective： To examine the effect of Insulin-like growth factor （IGF-1） on neurogenesis after focal cerebral ischemia in rats. Methods： Male SD rats were subjected to cerebral middle artery occlusion operation （MCAO）, and then they were randomly divided into three groups： sham-operation group, ischemia control group and IGF-1 group after successful modeling. Every 6 rats （random selected） in the three groups were sacrificed at 7d, 14d, 28d and 42d after MCAO, respectively. BrdU-labeled cells, PSA-NCAM-labeled cells, BrdU/PSA-NCAM-labeled cells expression, BrdU/MAP2-1abeled cells expression and BrdU/GFAP-labeled cells expression were identified by immunohistochemistry staining and double immunohistochemistry staining. Result： BrdU-ladeled cells and PSA-NCAM-labeled cells increased approximately by 4.0-fold and 1.8-fold compared with the ischemia control groups, 9.9-fold and 5.4-fold compared with sham-operated groups, respectively. Both BrdU-ladeled cells and PSA-NCAM-labeled cells reached the largest number at 7d after MCAO. BrdU/PSA-NCAM labeled cells expression were detected the largest cell number both in the SVZ and DG at 7d after ischemla and gradually decrease after that day. But BrdU/MAP：-labeled cells and BrdU/GFAP-labeled cells gradually increased from 14d after ischemia. Conclusion： IGF-1 induced cells proliferation before 7d and migration of regenerated cells at 7d-14d after MCAO operation in rats; differentiation of regenerated cells occurred while migration was proceeding from 14d after MCAO.

关 键 词：IGF-1 局灶脑缺血 神经发生 BRDU PSA-NCAM MAP2 GFAP 

分 类 号：R743.22[医药卫生—神经病学与精神病学]