人参皂苷Rh2诱导人胃癌SGC-7901细胞凋亡的研究  

Induction of Apoptosis of Human Gastric Carcinoma SGC-7901 Cells with Ginsenoside Rh2

作　　者：李晶华[1] 李杨[1] 何侃[1] 孔维[1] 金英花[1] 

机构地区：[1]吉林大学生命科学院

出　　处：《中国生物制品学杂志》2008年第1期45-46,50,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金项目(30640064;30770477)

摘　　要：目的研究人参皂苷Rh2(G-Rh2)对人胃癌细胞SGC-7901的影响。方法采用MTT法检测G-Rh2对SGC-7901细胞存活率的影响;流式细胞分析法检测凋亡小体的含量;免疫印迹技术及体外Caspase-3/-7活力测定方法检测Caspase的激活状态。结果G-Rh2对SGC-7901细胞生长有明显的抑制作用,且呈量-效关系,IC50为9.3μg/ml。7.5μg/mlG-Rh2作用SGC-7901细胞24h,凋亡细胞数量为6.97%。7.5μg/mlG-Rh2作用SGC-7901细胞20h,出现多聚(ADP-核糖)聚合酶[poly(ADP-ribose)polymerase,PARP]断裂,Caspase-3/-7活力开始出现,并随作用时间的延长而增强。结论G-Rh2诱导Caspase参与SGC-7901细胞凋亡。Objective To study the effect of ginsenoside Rh2（G-Rh2） on human gastric carcinoma SGC-7901 cells. Methods Add G-Rh2 to SGC-7901 cells to various final concentrations. Determine the survival rate of SGC-7901 cells by MTF method, the content of apoptotic body by flow cytometry, and evaluate the activation of caspase by Western blot and determination of caspase-3/-7 activity in vitro. Results G- Rh2 showed significantly dose-dependent inhibitory effect on the growth of SGC-7901 cells. The IC50 of G-Rh2 was 9.3 μg/ml. After treatment with 7.5 μg/ml of G-Rh2 for 24 h, the apoptosis rate of SGC-7901 cells was 6.97%. Both the cleavage of poly（ADP-ribose） polymerase （PARP）and caspase-3/-7 activity appeared after treatment with 7.5 μg/ml of G-Rh2 for 20 h and increased as time goes on. Conclusion G- Rh2 induced the apoptosis of SGC-7901 cells in which caspase was involved.

关 键 词：人参皂苷RH2 胃癌 SGS-7901细胞 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]