牛磺酸对高糖刺激大鼠视网膜Müller细胞GLAST和GS表达的影响  

Effect of taurine on expression of GLAST and GS in rat retinal Müller cells cultured with high glucose

作　　者：曾凯宏[1] 许红霞[1] 糜漫天[1] 张亚洁[1] 陈卡[1] 陈芳[1] 

机构地区：[1]第三军医大学营养与食品卫生学教研室,营养与食品安全重庆市市级重点实验室,中国重庆市400038

出　　处：《国际眼科杂志》2008年第1期41-44,共4页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30571570)

摘　　要：目的:通过检测牛磺酸对高糖刺激大鼠视网膜Müller细胞谷氨酸转运蛋白(glutamate-aspartate transporters,GLAST)和谷氨酰胺合成酶(glutamine synthetase,GS)表达的影响,探讨牛磺酸保护糖尿病引起视网膜损伤的机制。方法:高糖培养大鼠视网膜Müller细胞,分正常对照组(NC)、高糖组(high glucose,HG,25mmol/L)、高糖+0.1mmol/L牛磺酸(taurine,T)干预组(HG+0.1T)、高糖+1mmol/L牛磺酸干预组(HG+1T)、高糖+10mmol/L牛磺酸干预组(HG+10T)。用免疫荧光化学法和Western-blotting检测大鼠视网膜Müller细胞中GLAST和GS的表达。结果:高糖培养后,大鼠视网膜Müller细胞中GLAST和GS的表达明显减少(P<0.05),与高糖组相比,1mmol/L和10mmol/L牛磺酸干预可明显增加GLAST和GS的表达(P<0.05)。结论:牛磺酸增加Müller细胞中GLAST和GS的表达抑制糖尿病引起的视网膜Müller细胞功能改变。AIM ： To investigate the of glutamate-aspartate effect of taurine on expression transporters （GLAST） and glutamine synthetase （GS） in rat retinal Muller cells cultured with high glucose and observe the protection mechanism of taurine against diabetic retinopathy.METHODS： The rat retinal Muller cells cultured with high glucose were divided into normal control group, high glucose group and high glucose plus taurine of different dosage （0.1, 1, 10mmol/L taurine） group. The morphology was identified by confocal microscope. The changes of GLAST and GS expressed in Muller cells were studied with immunofluorescence staining method in a qualitative way and Western-blotting in a quantitative way. RESULTS： High glucose can significantly decrease the expression of GLAST and GS in Muller cells （P〈 0.05）. 1mmol/L and 10 mmol/L taurine intervention can significantly inhibit the down-regulation of GLAST and GS in Muller cells induced by high glucose（P〈0.05）. CONCLUSION： High glucose can down-regulate the expression of GLAST and GS in rat retinal Muller cells. Taurine can protect Muller cells from the damage of glucose.

关 键 词：牛磺酸 高糖 MULLER细胞 谷氨酸转运蛋白 谷氨 酰胺合成酶 

分 类 号：R774.1[医药卫生—眼科]