两种纳豆激酶活性测定方法对比及相关性分析  

A COMPARISON AND CORRELATION STUDY OF TWO METHODS FOR NATTOKINASE ACTIVITY DETECTION

作　　者：杨明俊[1] 杨晓彤[1] 冯慧琴[1] 糜可[1] 杨庆尧 

机构地区：[1]上海师范大学微生物与免疫学研究所,上海200234 [2]上海杨杨百草研究所,上海200234

出　　处：《食品研究与开发》2008年第2期137-141,共5页Food Research and Development

摘　　要：从线性和精密度两个方面对纤维蛋白平板法和四肽底物法测定纳豆激酶活性的两种方法进行了比较研究,并验证了两种方法测定值之间的直线相关性。结果表明:纤维蛋白平板法和四肽底物法分别在10.77IU/mL～80.73IU/mL和0.03U/mL～0.09U/mL浓度范围内呈线性,且日间和日内变异系数均小于10%。两者结果具有直线相关性,其关系为纤维蛋白平板法测定值(IU)=264.87×四肽底物法测定值(U)-19.633,(R2=0.9942)。结论:纤维蛋白平板法与四肽底物法均能用于纳豆激酶活性测定,前者测定结果直接,但四肽底物法更灵敏,还可应用于高通量筛选。This study compared the linearity and precision of fibrin plate （FP） method with tetra-peptide （Suc-Ala-Ala-Pro-Phe-pNA） substrate （SS） method, and their results＇ correspondency was also investigated. Results showed that： The activity linear ranges were 10.77 IU/mL-80.73 IU/mL for FP method and 0.03 U/mL- 0.09 U/mL for SS method respectively, while both CVs were less than 10% in within-day and day-to-day tests. The two results showed a good linear correlation and could be convened as： FP values （IU） = 264.87 x SS values （U） - 19.633, （R2 = 0.994 2）. Therefore： fibrin plate method and tetra-peptide substrate （SS） method are both applicable for detecting Nattokinase activity. The result from FP method can directly reflect nattokinase＇s thrombolytic activity and the tetra-peptide substrate method has the advantage for fast, sensitive and high-throughput screening.

关 键 词：纳豆激酶 纤维蛋白平板法 四肽底物法 

分 类 号：TS201.25[轻工技术与工程—食品科学]