反相高效液相色谱同步对酸橙汁中柚皮柑和橙皮柑的定量分析研究  

Quantitative Analysis of Narigin and Hesperidin contents in Citrus Aurantium Juice by Reverse-phase High-performance Liquid Chromatography

作　　者：何建新[1] 张群[1] 林树花[1] 

机构地区：[1]湖南省农产品加工研究所,湖南省食品测试分析中心,湖南长沙410125

出　　处：《现代食品科技》2008年第1期83-85,共3页Modern Food Science and Technology

基　　金：湖南省“十一五”重大专项《现代柑桔产业化关键技术研究与示范项目》(NO.2006NK1002)

摘　　要：研究了RP-HPLC同步测定酸橙汁中的柚皮柑和橙皮柑法的色谱条件,并对甲醇溶解+超声处理与水溶解+静置处理进行比较,并对结果进行准确度和精密度试验。其色谱条件为V甲醇:V水=40:60,固定相ODS-C18,柱子4.6μm×150mm,流速1mL/min,检测波长285nm;甲醇溶解+超声处理测得的酸橙汁中柚皮苷、橙皮苷含量高于水溶解+静置处理,含量分别提高了32%和4.4%;试验操作简便,结果准确可靠。The chromatographic conditions for quantitative analysis of narigin and hesperindin contents in citrus aurantium juice were investigated and the optimal reverse-phase chromatogram conditions were found as follows： methanol/water being of 40/60, column being of ODS-C18 （4.6μm× 150mm）, flow rate of 1 mL/min and the detection wavelength being of 285nm. It was also found that the contents of narigin and hesperidin in citrus aurantium juice via methanol dissolve and supersonic treatment were 32% and 4.4% higher than those via water dissolve and standing storage, respectively. The method was simple and accurate .

关 键 词：反相高效液相色谱 同时测定 酸橙汁 柚皮柑 橙皮柑 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]