聚合酶链反应检测生殖支原体的研究  

Detection of Mycoplasma Genitalium by Polymerase Chain Reaction

作　　者：张伟云[1] 王荷英[1] 叶顺章[1] 施美琴[1] 王红春[1] 

机构地区：[1]中国医学科学院,中国协和医科大学皮肤病研究所,南京210042

出　　处：《中华皮肤科杂志》1997年第3期170-171,共2页Chinese Journal of Dermatology

摘　　要：具有致病性的生殖支原体（Ｍｇ）生长缓慢，不易培养，而血清学诊断与肺炎支原体又有交叉反应，这给Ｍｇ的临床诊断带来困难。为了进一步解决Ｍｇ的临床检测问题，本研究采用Ｍｇ粘附因子基因序列合成一对引物ＭｇＰａ１和ＭｇＰａ３，用聚合酶链反应（ＰＣＲ）方法检测Ｍｇ。结果表明，对Ｍｇ能产生特异性扩增，片段大小为２８１ｂｐ，而对肺炎支原体、解脲支原体、人型支原体、沙眼衣原体及淋球菌等则不能扩增出任何片段。作者认为，ＰＣＲ为一种高度特异和敏感性的方法，它为Ｍｇ的临床检测提供了重要的手段。我们还对引物浓度、退火温度及模板量对ＰＣＲ扩增结果的影响进行了初步探讨。Pathogenic Mycoplasma genitalium multiplies slowly and is difficult to be cultured. Furthermore, M.genitalium(Mg) and M.pneumoniae share common antigens, which can give rise to extensive cross reactions in serological tests, this makes the diagnosis of Mg infection difficult. In order to solve this problem, we detected Mg by polymerase chain reaction(PCR) method. On the basis of the published nucleotide sequence of M. genitalium adhesion protein gene,two primers,MgPa 1 and MgPa 3,were synthesized. The results showed that amplification of Mg DNA yielded the predicted 281bp fragment, and amplification of M.pneumoniae, U.urealyticum,M.hominis,C.trachomatis and N.gonorrhoeae etc did not yield any fragment. It is the authors′, opinion that PCR is specific and sensitive, and it provides an important method for the laboratory diagnosis of Mg infection.The effects of primer concentration, annealing temperature and quantity of template on the result of amplification were discussed.

关 键 词：生殖支原体 聚合酶链反应 NGU 尿道炎 诊断 

分 类 号：R691.304[医药卫生—泌尿科学] R446.5[医药卫生—外科学]