大豆小分子肽抗疲劳效果评价  

Evaluation on anti-fatigue effect of soybean minor peptides

作　　者：闫萍[1] 李茂辉[2] 郭红[1] 高淑杰[3] 

机构地区：[1]沈阳医学院第三附属医院,沈阳110034 [2]沈阳医学院预防医学系卫生检验中心 [3]沈阳体育学院运动生化教研室

出　　处：《中国公共卫生》2008年第2期217-218,共2页Chinese Journal of Public Health

基　　金：沈阳市科技局基金项目(1053125-1-39)

摘　　要：目的通过动物实验研究大豆小分子肽（分子量300～700Da）的抗疲劳作用。方法将昆明种小鼠分为低、中、高大豆小分子肽（3，6，9g／kg／d）3个剂量组灌胃25d，测定小鼠的力竭游泳时间以及耐缺氧能力。将Wister大白鼠，按同样剂量分组大豆小分子肽灌胃50d，测血红细胞和肝脏中超氧化物歧化酶（SOD）活力。同时测定2个实验组（各设一个生理盐水灌胃对照组）肝糖原、血乳酸、血尿素氮。结果大豆小分子肽（分子量300～700Da）的中、高剂量组小鼠负重游泳时间明显延长。高剂量组具有明显耐缺氧能力。并且高剂量组小鼠运动后血乳酸含量与对照组比较明显降低。肝糖原和血尿素氮含量与对照组比较差异无统计学意（P〉0．05）。而中、高剂量组的大鼠的血红细胞SOD活力明显高于对照组（P〈0．05），而肝脏组织SOD酶活性有升高趋势，但差异无统计学意义（P〉0．05）。结论大豆小分子肽（分子量300～700Da）具有一定增强小鼠抗疲劳和耐缺氧的能力。Objective To study anti - fatigue effect and biological mechanism of soybean minor peptides （MW300 700Da）. Methods Soybean minor peptides was administered to mice in 3 treated groups for 25 days by 3.0, 6.0, 9.0 g/kg·d）respectively, and the changes of exhaustive swimming time and the bear anoxia ability were measured. Soybean minor peptides was administered to Wister mice in same treated group for 50 days, the activities of superoxide dismutase（SOD） of Hb and liver were measured. Then biochemical index including liver glycogen, blood lactic acid, urea nitrogen were measured in 2 treated groups（One is physiological saline controigroup）. Results Compared with control group, the exhaustive swimming time and bear anoxia ability of administered mice in middle and high dosage group were significantly longer and blood lactic acid content obviously reduces in high dosage group. And there is no significant difference in liver glycogen and urea nitrogen （P 〈 0.05）. While the SOD activities of Hb in centre and high dosage group mice obviously increased （P 〈 0.05 ）. And the SOD activities of liver in mice increased, but without significant difference （P 〉 0.05） between groups. Conclusion The soybean minor peptides （MW300- 700Da） have a potential anti- fatigue effect and the bear anoxia ability in mice.

关 键 词：大豆小分子肽 抗疲劳 力竭游泳时间 耐缺氧能力 超氧化物歧化酶 

分 类 号：R151.2[医药卫生—营养与食品卫生学]