双波长比值光谱法测定5'-鸟苷酸钠、5'-肌苷酸钠、5'-尿苷酸钠含量  

Determination of Guanosine 5'-monophosphate, Inosine 5'-monophosphate and Uridine 5'-monophosphate by Dual-wavelength Ratio Spectrometry

作　　者：杜建中[1] 杨春梅[1] 李自信[1] 冯世生[1] 李翠[1] 

机构地区：[1]湛江师范学院化学科学与技术学院,广东湛江524048

出　　处：《食品科学》2008年第1期225-227,共3页Food Science

摘　　要：依据5'-鸟苷酸钠、5'-肌苷酸钠和5'-尿苷酸钠三组分的比值光谱特征,以尿苷酸钠为干扰组分时,选择258、285、244、280nm作为测定鸟苷酸钠和肌苷酸钠的波长;以肌苷酸钠为干扰组分时,选择211、241nm作为测定尿苷酸钠的波长。结果显示鸟苷酸钠在0.15～5mg/100ml,肌苷酸钠在0.2～6mg/100ml,尿苷酸钠在0.25～4mg/100ml范围具有良好的线性关系,平均回收率分别为100.2%、100.2%和99.5%。本法具有测定波长少、计算简单、光谱"分离"能力强以及能在低档分光光度计上实现、易于推广等特点。According to the respective features of the ratio spectra of the guanosine 5＇-monophosphate（5＇-UMP）, inosine 5＇- monophosphate（5＇-IMP） and uridine 5＇-monophosphate（5＇-UMP） and taking 5＇-UMP as disturbance component, 258, 285, 244 and 280 nm were chosen as the determining wavelengths of the 5＇-GMP and 5＇-IMP. Taking the 5＇-IMP as disturbance component, 211 and 241 nm were chosen as the determining wavelengths of the 5＇-UMP. It showed that for the three components good linear correlationships exit as 5＇-GMP（0.15 -5 mg/100 ml）, 5＇-IMP（0.2-6 mg/100 ml）, 5＇-UMP （0.25-4 mg/ 100 ml）. The average recoveries are 100.2%, 100.2% and 99.5% respectively. The method is simple and practical with fewer determination wavelengths and powerful spectral resolution and can be performed on a lower level instrument. It is easy to generalize application.

关 键 词：双波长比值光谱法 5'-鸟苷酸钠 5'-肌苷酸钠 5'-尿苷酸钠 

分 类 号：R917[医药卫生—药物分析学]