绿茶及其有效成分对肾性高血压大鼠左室肥厚抑制作用的研究  

Molecular mechanism by which green tea and tea extract inhibits left ventricle hypertrophy induced by renovascular hypertension in rats

作　　者：毛伟峰[1] 宋雁[1] 韩驰[1] 李宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《卫生研究》2008年第1期43-46,共4页Journal of Hygiene Research

基　　金：国家科技部"十一五"支撑项目(No2006NAK02A07)

摘　　要：目的研究绿茶及其提取物对肾性高血压大鼠左室肥厚的抑制作用机制。方法雄性Wistar大鼠160只,体重180-220g,随机分为5组,建立二肾一夹法大鼠肾性高血压左室肥厚模型：假手术组（SHAM）和手术组（2K1C）正常饮水;手术＋绿茶组（2K1C＋GT）饮用2%绿茶水;手术＋茶多酚组（2K1C＋TP）饮用0.1%茶多酚水;手术＋EGCG组（2K1C＋EGCG）饮用0.05%EGCG水,术后即给予不同浓度的绿茶及其提取物,持续8周直至实验结束。结果与假手术组比较,手术组和各饮茶组血压、左心室与体重比值（LVW/BW）和左心室壁厚度（LVWT）均显著升高（P〈0.01）。而与手术组相比,不同浓度的绿茶及其有效成分（2%绿茶、0.1%茶多酚、0.05%EGCG）左心室重/体重（LVW/BW）、左心室壁厚度显著降低,抗氧化酶（SOD和GSH-Px）的活性升高,活性氧自由基（ROS）以及Ras和ERK1/2蛋白表达降低,但对大鼠收缩压无显著性影响。结论绿茶及其提取物对肾性高血压大鼠左室肥厚有抑制作用,其机制可能是由于茶及其有效成分具有抗氧化活性,能够清除ROS,以及对Ras-MAPK信号传导通路的调控有关。Objective To investigate the preventive effects and the molecular mechanism of tea and tea extracts （polyphenols and EGCG） on left ventricle hypertrophy （LVH） induced by Renovascular hypertension in rats. Methods One hundred and sixty male Wistar rats were randomly divided into five groups： negative control group （SHAM）, operation group （2K1C）, green tea group （2KIC ＋ GT）, tea polyphenol group （2K1C ＋ TP）, EGCG group （2K1C ＋ EGCG）. After surgery operation, rats in different groups received tap water, tap water, 2% green tea, 0.1% tea polyphenol and 0.05 % EGGG respectively, as the sole drinking source for 8 weeks until the end of the experiment. Results The results showed that the blood pressure, the ratio of left ventricle weight to body weight（LVW/BW） and the left ventricular wall thicknesses （LVWT） in 2K1C group significantly increased（ P 〈 0.01 ）, when compared to those in sham operation group. However, when compared to these parameters in operation group, the ratio of LVW/BW and HW/BW in green tea group, tea polyphenols group and EGCG group significantly decreased （P 〈 0.05 ）, while the activities of GSH-Px and SOD increased, and the levels of ROS in heart were also significantly decreased（ P 〈 0.05）. Ras, P-ERK protein expressions in tea-treatment groups were also decreased. Conclusion Green tea, tea polyphenols and EGCG can attenuate the development of left ventricular hypertrophy induced by renal hypertension in rats. The possible mechanisms may be due to its antioxidant properties and the modulation of Ras-to-MAPKs mediated signal transduction.

关 键 词：绿茶及其提取物 肾性高血压 左室肥厚 二肾一夹 绿茶 

分 类 号：R544.14[医药卫生—心血管疾病] TS272.51[医药卫生—内科学]