6-O-羧甲基氨基葡萄糖的制备及其结构分析  

Preparation and structural analysis of C_6-CM-glucosamine

作　　者：常菁[1] 刘万顺[1] 韩宝芹[1] 王常红[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《海洋科学》2008年第2期63-68,共6页Marine Sciences

基　　金：国家863计划资助项目(2003AA625050)

摘　　要：采用酶降解和酸降解相结合的工艺,将大分子量的6-O-羧甲基甲壳素降解成单分子的6-O-羧甲基氨基葡萄糖,经906型弱碱性阴离子交换树脂柱吸附层析分离,制得羧甲基化氨基糖。对该单糖进行了HPLC、高效薄层、比旋光度、熔点和红外光谱等方面的分析研究。结果表明,该单糖在C6连有羧基,C2连有氨基,具有特定的比旋光度和熔点特征,与报道的结果相一致。By use of both enzymolysis and acid hydrolysis, the 6-O-CM-chitin with high MW could be degraded to 6-O-CM-glucosamine, which is a monosaccharide. Then we studied its HPLC, HTLC, optical rotation, melting point, IR imagery and so on. The result shows that this monosaccharide has a carboxyl group on C6, an amino group on C2 and also has its own optical rotation and melting point, which are just in accordance with the results reported.

关 键 词：壳聚糖酶 溶茵酶 6-O-羧甲基氨基葡萄糖 酸水解 

分 类 号：Q532.5[生物学—生物化学]