脂肪酶假单胞菌的分离培养及最佳产酶条件研究  

Study on Isolating Culture of Lipase Pseudomonad/Pseudomonas and Its Optimal Producing Condition of Enzyme

作　　者：周海霞[1] 袁丽红[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《现代生物医学进展》2008年第2期259-262,共4页Progress in Modern Biomedicine

基　　金：国家重大基础研究项目(No.2003CB716000);国家自然科学基金重点项目(No.20336010)资助

摘　　要：以麻疯树油为唯一碳源,从以粉碎的麻疯树种子处理过的土壤中分离筛选出1株脂肪酶活性较高的茵株,初步鉴定为假单胞菌属(Pseudomonas)。实验观察了碳源、氮源、无机盐及发酵工艺对产酶的影响,摇瓶发酵结果表明,该菌株最适产酶培养基的组成是(%,w/v):橄榄油2,酵母膏0.5,(NH_4)_2SO_40.5,MgCl_2·6H_2O 0.5,最适产酶温度为30℃,最佳产酶pH为6.5,转速180r/min,发酵培养36h酶活达到最高,为14.17U/mL。本研究为以麻疯树油为原料酶法生产生物柴油奠定了一定的基础。A wild lipase-producing strain was isolated from the soil pretreated by Jatropha curcas seed powder with Jatropha oil as sole carbon source and identified as Pseudomonas sp. The fermentation conditions of the lipase-production strain were optimized and the effects of carbon sources, nitrogen sources, and fermentation techniques on the lipase production were studied. The results showed that the optimal medium composition for lipase production was as follows （%, w/v）： olive oil 2.0, yeast extract 0.5, （NH4）2SO4 0.5,MgCl2· 6H2O 0.5. The optimal initial pH of fermentation medium was 6.5. The maximum yield was 14.17 U/mL and obtained when Pseudomonas sp, was shake-cultivated at 30℃ for 36 h with rotation speed of 180r/min.

关 键 词：脂肪酶 假单胞菌 分离 培养 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]