Notch1信号蛋白在卵巢癌细胞系中的表达  

Expression of Notchl Signal Protein in Ovarian Carcinoma Cell Lines

作　　者：王明义[1] 辛晓燕[1] 刘玉[1] 

机构地区：[1]第四军医大学西京医院妇产科,陕西西安710033

出　　处：《现代生物医学进展》2008年第2期270-271,共2页Progress in Modern Biomedicine

摘　　要：目的:检测卵巢癌细胞系中Notch1信号蛋白的表达,为在卵巢癌细胞中对Noah1进行RNA干涉研究的体外实验筛选靶细胞。方法:采用逆转录聚合酶链反应(RT-PCR)法及Western blotting对卵巢癌细胞系SKOV3、HO-8910、HO-8910-PM和3AO中Notch1进行检测。结果:Notch1信号蛋白在4种卵巢癌细胞系中均有表达,其在HO-8910-PM中表现为高表达,在SKOV3和3AO中表现为中等表达,在HO-8910中表现为低表达。结论:HO-8910-PM适合作为靶细胞,进行Notch1信号蛋白的RNA干涉研究。Objectives： To screen target cells for the study of RNA interference targeting at Notch 1 by detection the expression of Notchl gene in ovarian carcinoma cell lines. Methods： The expressions of Notchl in SKOV3, HO-8910, HO-8910-PM and 3AO cell lines were examined by using RT-PCR analysis and Western blotting. Results： The expression ofNotchl mRNA and protein could be examined in all four ovarian carcinoma cell lines. The expression of Notchl is strong positive expression in HO-8910-PM cell line, positive expression in SKOV3 and 3AO cell lines and weak positive expression in HO-8910 cell line. Conclusion： HO-8910-PM cell line could be used as target cells for the study of RNA interference targeting at Notch 1 in ovarian carcinomas.

关 键 词：卵巢癌细胞系 NOTCHL 逆转录聚合酶链反应 WESTERN BLOTTING 

分 类 号：R737.31[医药卫生—肿瘤]