鼻咽癌细胞系5-8F和6-10B的差异蛋白质组学研究  

Differential proteomic analysis of naspharyngeal carcinoma cell lines 5-8F and 6-10B

作　　者：向亚莉[1] 易红[1] 李萃[1] 张鹏飞[1] 李茂玉[1] 陈主初[1] 肖志强[1] 

机构地区：[1]中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室

出　　处：《中南大学学报（医学版）》2007年第6期978-984,共7页Journal of Central South University ：Medical Science

基　　金：国家重点基础研究发展计划(973)资助项目(2001CB510207);湖南省科技厅重点科研项目(06SK2004)~~

摘　　要：目的:比较不同转移潜能的鼻咽癌(nasopharyngeal carcinoma,NPC)细胞系5-8F和6-10B细胞蛋白质组的差异,筛选NPC转移相关蛋白质。方法:应用二维凝胶电泳(two-dimensional gel electro-phoresis,2-DE)技术分离具有相同遗传背景、不同转移潜能的鼻咽癌细胞系5-8F和6-10B细胞的蛋白质,图像分析识别差异表达蛋白质点,基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorp-tion/ionization time-of-flight mass spectrometry,MALDI-TOF-MS)鉴定差异表达的蛋白质,Western免疫印迹方法验证部分差异蛋白质在2株细胞中的表达水平。结果:建立了5-8F和6-10B细胞蛋白质的2-DE图谱,识别了65个差异表达的蛋白质点,鉴定了15个非冗余的差异表达蛋白质。Western免疫印迹分析证实了差异蛋白质膜联蛋白A1和14-3-3protein sigma在5-8F和6-10B细胞中的差异表达水平。结论:15个非冗余的差异表达蛋白质为研究NPC转移机制提供了实验依据。Objective To compare the proteome difference of nasopharyngeal carcinoma（NPC）cell lines 5-8F and 6-10B,and to screen these proteins associated with NPC metastasis.Methods Two-dimensional gel electrophoresis（2-DE）was used to separate the total proteins from NPC cell lines 5-8F and 6-10B with different metastatic potentials and same genetic background,respectively.PDQuest software was applied to analyze 2-DE images,and the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS）.The expression levels of partial identified proteins in the 2 cell lines were detected by Western blot.Results 2-DE maps of total proteins from 5-8F and 6-10B were established.A total of 65 differential protein spots in the 2 cell lines were found,and 15 non-redundant differential expression proteins were identified by MALDI-TOF-MS.Western blot showed that Annexin A1 and 14-3-3 protein sigma were differential expression proteins in 5-8F and 6-10B,which was consistent with the results from the comparative proteomic analysis.Conclusion Fifteen non-redundant differential expression proteins are useful for studying the metastatic mechanism of NPC.

关 键 词：鼻咽癌 转移 双向凝胶电泳 质谱 

分 类 号：R739.6[医药卫生—肿瘤]