原花色素对HepS细胞增殖抑制诱导凋亡的研究  

Growth Inhibition and Apoptosis Induction of Liver Cancer HepS Cells Line by Proanthocyanidins

作　　者：彭晓光[1] 张太平[1] 彭士明[1] 张鹤云[1] 

机构地区：[1]南京大学生命科学院医药生物技术国家重点实验室,南京210093

出　　处：《天然产物研究与开发》2007年第B08期233-236,共4页Natural Product Research and Development

摘　　要：本文通过体外培养肝癌HepS细胞，以不同浓度原花色素处理12—72h后，MTT法测定细胞生长抑制作用，采用DNA片断分析、DNA琼脂糖凝胶电泳、荧光染色以及流式细胞技术等方法来探讨原花色素体外抑制肝癌HepS细胞及诱导其凋亡的作用。实验结果显示原花色素能抑制HepS细胞的生长，并且呈现出明显的时效和量效关系，DNA电泳出现典型的凋亡DNA梯形带，在荧光显微镜下，凋亡细胞呈亮绿色，H和AnnexinV．FIFC双染后，经流式细胞仪检测、分析显示凋亡细胞明显增多。因此原花色素能抑制肝癌HepS细胞株的生长，可能与诱导其细胞凋亡有关。Proanthocyanidins are of current interest as anticancer agent, Liver cancer HepS cells line were treated with proanthocyanidins with different concentrations for 12-72 h in vitro. Through the method of MTT assay, DNA fragment, DNA agarose gel electrophoresis, and flow cytometric analysis, we can investigate the effect of inhibition and apeptosis induction of proanthocyanidins on HepS cells line in vitro. MTT assay was used to measure the effect of the growth inhibition and apeptosis of the cells were observed by DNA fragment, DNA agarose gel electrophoresis, and flow cytometric analysis of HepS cells. The results unraveled that proanthocyanidins could inhibit the growth of the cells and present the dependent relationship of time and concentration in the range of time and dose. DNA of the apeptosis ceils displayed the DNA ladder tapes by DNA agarose gel electrophoresis. The flow cytometric analysis of HepS cells by Annexin V-FIFC and propidium iodide double staining demonstrated that quantity of apeptosis cell increased with proanthocyanidins induction. It may be one of the anticarcinogenic mechanisms that proanthocyanidins could inhibit the growth of the liver cancer HepS cells and induce cells apeptesis.

关 键 词：原花色素 HEPS Hoeehest33342 细胞凋亡 DNA琼脂糖凝胶电泳 

分 类 号：R739[医药卫生—肿瘤]