虫草制品中腺苷和虫草素含量的RP-HPLC分析  

Determination of Adenosine and Cordycepin in Cordyceps Products by RP-HPLC

作　　者：邢增涛[1] 李光平[1] 孟范贵[2] 谢文明[2] 门殿英[1] 

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所,农业部食用菌产品质量监督检验测试中心（上海）,上海201106 [2]吉林农业大学资源与环境学院,长春130118

出　　处：《天然产物研究与开发》2007年第B08期256-259,共4页Natural Product Research and Development

基　　金：上海市科学委员会局管项目（04JG05048）

摘　　要：采用超声提取，反相高效液相色谱方法分析了各种虫草制品中的腺苷和虫草素的含量，以含0．05mol／L 的磷酸二氢钾：甲醇（85／15，V／V）作为流动相。检测波长为260nm。进样量为10μL，40℃条件下检测。在5mg／L到250mg／L的浓度范围内呈线性相关。研究显示利用蒸馏水在60℃超声提取20min，腺苷和虫草素的回收率在85％-95％之间。A new RP-HPLC method has been developed for the simultaneous determination of 2 main nucleosides ingreclients, including adenosine and cordycepin in Gordyceps. Determination was achieved on a Diamonsil C18 analytical colunto （4.6×250 mmid, 5μm） using methanol water mobile phase elution with UV detection. Both calibration curves showed good linearity （R^2 〉 0.9995） within the test ranges from 5 to 250 mg/L. The developed method was simple, rapid and accurate, and showed good reproducibility for the quantification of 2 nucleosides in natural and cultured Cordyceps products with an inter-day variations of less than 3.83% and 2.28% for adenosine and cordycepin, respectively.

关 键 词：蛹虫草 腺苷 虫草素 反相高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]