莠去津降解菌HB-5的最佳产酶培养基及发酵条件  

Optimized culture medium and fermentation conditions for enzyme production by the atrazine-degrading bacterium HB-5

作　　者：王倩[1] 朱鲁生[1] 王军[1] 谢慧[1] 刘伟[1] 马婷婷[1] 董晓丽[1] 

机构地区：[1]山东农业大学资源与环境学院,泰安271018

出　　处：《环境科学学报》2008年第2期299-304,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No20477022);山东省优秀中青年科学家科研奖励基金(No03BS123)~~

摘　　要：从农药厂废水中分离到一株降解莠去津的节杆菌(Arthrobacter sp.)HB-5,以从该菌中提取到的降解酶对莠去津的降解率为指标,进行最佳产酶培养基及发酵条件的优化研究,对其产酶量进行了评价.通过正交试验和均匀试验,对细菌HB-5的发酵培养基进行了优化研究.运用SAS软件进行结果分析,所获优化培养基配方为:蔗糖3.0g·L-1,莠去津0.38g·L-1,K2HPO40.5g·L-1,KH2PO41.2g·L-1,MgSO4.7H2O1.2g·L-1,NaCl0.1g·L-1,微量元素溶液3.8mL·L-1.得到菌株培养的最佳优化条件为:菌株发酵液培养时间为48h,接种量为2%,发酵液初始pH值为9,250mL三角瓶中装液量为80mL.经优化后,降解酶对莠去津的降解率(91.64%)比原培养基(40.67%)提高了125%.An atrazine degrading bacterium （Arthrobacter sp.） HB-5, was isolated in our laboratory from pesticide waste water. The optimum fermentation conditions and fermentation culture medium of HB-5 were selected by uniform experimental design and orthogonal experimentation, and the amount of enzyme produced by HB-5 was estimated from the atrazine degradation rate. The experimental results were analyzed by the SAS system. The ideal composition of culture medium of HB-5 included saccharose 3.0 g·L^-1, atrazine 0.38 g·L^-1, K_2 HPO_4 1.2 g·L^-1, K_2 HPO_4 0.5 g·L^-1, MgSO_4·7 H_2O 1.2 g·L^-1, NaCl 0.1 g·L^-1, microelements 3.8 mL·L^-1. The optimum culture conditions of HB-5 were ： culture time 48 h, inoculum size 2% , initial pH 9.0 and amount of culture medium 80 mL. The degradation rate of atrazine by the optimum fermentation liquid reached up to 91.64% , and increased 125% over the original culture medium （40.67%）.

关 键 词：莠去津 发酵 细菌HB-5 降解率 

分 类 号：X171[环境科学与工程—环境科学]