Effect and mechanism of homocysteine on Parkinson's disease induced by 6-OHDA  

作　　者：Hongxia Xing Hai Peng Xuebing Cao Shenggang Sun 

机构地区：[1]Department of Neurology, The First Hospital of Xinxiang Medical College, Xinxiang 453100, Henan Province, China [2]Department of Neurology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China

出　　处：《Journal of Nanjing Medical University》2008年第1期12-17,共6页南京医科大学学报（英文版）

基　　金：National Natural Sciences Foundation of China(No.30300114)

摘　　要：Objective： To study the effects and mechanism of homocysteine（Hcy） on Parkinson＇ s disease（PD） induced by 6-hydroxydopamine （6-OHDA） in vivo. Methods：Forty rats were divided into 4 groups. 6-OHDA or the solvent of 6-OHDA was focally administrated to induce PD, 2 h later Hcy or 0.9% sodium chloride was administrated in the ipsolateral substantial nigra（SN）. We Used behavioral testing, Immolunohistochemical techniques, biochemistry techniques to detect the injury of SN. Results：The rotary turns of PD rats induced by 6-OHDA showed significant increase after treatment with Hcy compared with the controls（P 〈 0.05）. Also the numbers of tyrosine hydroxylase（TH）-stained neurons were decreased, and dendrites were fragmented and truncated. Free radicals were increased and antioxidaat enzymes decreased. Conclusion：Focal infusion of Hcy into the SN increased the vulnerability of the doparninergic neurons to 6-OHDA-induced degeneration, it seems that the endangering effect of Hcy is due to exacerbating oxidative stress.Objective： To study the effects and mechanism of homocysteine（Hcy） on Parkinson＇ s disease（PD） induced by 6-hydroxydopamine （6-OHDA） in vivo. Methods：Forty rats were divided into 4 groups. 6-OHDA or the solvent of 6-OHDA was focally administrated to induce PD, 2 h later Hcy or 0.9% sodium chloride was administrated in the ipsolateral substantial nigra（SN）. We Used behavioral testing, Immolunohistochemical techniques, biochemistry techniques to detect the injury of SN. Results：The rotary turns of PD rats induced by 6-OHDA showed significant increase after treatment with Hcy compared with the controls（P 〈 0.05）. Also the numbers of tyrosine hydroxylase（TH）-stained neurons were decreased, and dendrites were fragmented and truncated. Free radicals were increased and antioxidaat enzymes decreased. Conclusion：Focal infusion of Hcy into the SN increased the vulnerability of the doparninergic neurons to 6-OHDA-induced degeneration, it seems that the endangering effect of Hcy is due to exacerbating oxidative stress.

关 键 词：Parkinson＇s disease 6-HYDROXYDOPAMINE HOMOCYSTEINE tyrosine hydroxylase 

分 类 号：R98[医药卫生—药品]