扩展青霉脂肪酶的生产及其在外消旋烯丙醇酮拆分上的应用  

Production of the Lipase from Penicillium expansum PED-03 and its Application in the Resolution of Racemic Allethrolone

作　　者：戴大章[1] 夏黎明[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,浙江杭州310027

出　　处：《高校化学工程学报》2008年第1期166-171,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：中国博士后科学基金(20060401045)

摘　　要：试图应用脂肪酶催化拆分法代替化学法拆分外消旋烯丙醇酮(4-羟基-3-甲基-2-(2-烯丙基)-2-环戊烯-1-酮,allethrolone)。以Rodamine B平板筛选法获得了一株脂肪酶高产菌Penicillium expansum PED-03,并对该菌液态发酵产脂肪酶的主要工艺参数进行了优化。确立了0.5%的淀粉和4%的豆饼粉为该菌种的适宜碳源和氮源,最适C/N为0.5。Tween-80对产酶有明显的促进作用,添加0.05%Tween-80后所产酶活力可提高55%。发酵罐产酶试验结果表明,搅拌速度对脂肪酶的产生有明显影响,适宜的搅拌速度为500r·min·1。利用P.expansum PED-03脂肪酶在非水相中对外消旋烯丙醇酮进行手性拆分,反应36h时转化率(C)可达理论值的96%,产物的对映体过量值(eeP)可达99%,显示了该酶在烯丙醇酮的酶法拆分方面具有良好的应用潜力。The resolution of racemic allethrolone （4-hydroxy-3-methyl-2-（2-propenyl）-2-cyclopenten-l-one） catalyzed by lipase instead of chemical methods was investigated. A high-yield lipase producing strain Penicillium expansum PED-03 was obtained by Rhodamine B plate screen. It is suitable for the lipase production with 0.5% starch as carbon source and 4.0% soybean meal as nitrogen source in submerged fermentation. Tween-80 is an effective enhancer for lipase production, and the lipase activity can be increased 55% when 0.05% Tween-80 was added to the medium. Agitation speed plays an important role in the lipase production, and the suitable agitation speed is 500 r·min^-1. The resolution of racemic allethrolone catalyzed by the lipase from P. expansum PED-03 was carried out in a nonaqueous medium, and after reacting for 36 hours, the conversion reaches 96% of its theoretical value and the enantiomeric excess of product （eep） reaches 99%. It shows that the enzymatic resolution method proposed presents a good promise of using it for the reslution of racemic allethrolone.

关 键 词：脂肪酶 液态发酵 酶法拆分 烯丙醇酮 

分 类 号：Q556[生物学—生物化学] Q814.9