牛磺酸抑制二甲基苯蒽诱发大鼠乳腺癌及其机制研究  

STUDY ON THE ANTICANCER ACTION OF TAURINE ON 7,12-DIMETHYLBENZ (a) ANTHRACENE (DMBA) INDUCED BREAST-CANCER IN RATS

作　　者：薛美兰[1] 张华荣 姜长青[3] 张秀珍[1] 

机构地区：[1]青岛大学医学院,青岛266021 [2]青岛卫生学校,青岛266021 [3]青岛市立医院,青岛266021

出　　处：《营养学报》2008年第1期57-60,共4页Acta Nutrimenta Sinica

基　　金：山东省卫生厅科研基金(No.HZ008)

摘　　要：目的观察牛磺酸(Tau)对二甲基苯蒽(DMBA)诱发大鼠乳腺癌的影响及其机制。方法7w龄雌性Wistar大鼠分为A、B、C(饮水中分别添加0.5%、1.0%、1.5%Tau)、D(正常对照组)和E(肿瘤对照组)5组。A、B、C、E组皮下一次注射DMBA10mg/100gbw;D组注射等量溶剂。实验期18w,观察体重(BW)、生长发育及肿瘤发生情况。实验结束时处死全部动物,剥离肿瘤组织及脏器,检测血清抗氧化指标、DNA损伤代谢产物及免疫学指标。结果三组肿瘤发生率A(53.33%)、B(46.67%)、C(40.40%)逐渐降低,其中C组明显低于E组(82.35%)。肿瘤潜伏期以E组最短,A、B和C组较长。平均瘤重和瘤体比:B和C组明显低于E组。与D组比,各组动物脾脏脏体比A组稍高,胸腺脏体比E组最小,与B、C、D组差别显著。C组血淋巴细胞增殖率高于E组,脾淋巴细胞增殖指数A组高于D和E组。与E组比,A、B、C和D组的血清SOD活力明显升高,MDA生成有所降低,而GSH-Px活力差别不明显。A、B、C和D组的血浆O6-甲基鸟嘌呤含量较E组明显降低。结论牛磺酸对大鼠诱发乳癌的发生和生长有较明显的抑制作用,是通过增强机体免疫、抗氧化作用、增强DNA损伤修复、抑制肿瘤细胞增殖等多种途径实现的。Objective To investigate the anticancer action of taurine（Tau） on 7, 12-Dimethy- lbenz （a） anthracene （DMBA） induced- tumor in Wistar rats. Method Seven-week-old female rats were randomly divided into five groups： group A,B,C （0.5%, 1.0% , 1.5% Tau respectively）; group D （normal control）; group E （tumor control）. One week after Tau supplementation, 10 mg/100g DMBA were subcutaneously given to each rat except group D. The trial lasted 18 w. By 6 w after DMBA administration the rats were palpated once weekly and the time, size, location of each tumor were recorded. At the end of trial, T-cell proliferation rates and indices, serum SOD, MDA, GSH-Px and O^6-MeG were determined. Results The tumor incidence in group A, B and C was gradually decreased, being 53.33%, 46.67%, 40.40% respectively, group C obviously lower than group E （82.35%）. The latent period of tumor was shortest in group E and was prolonged in group A, B and C significantly. The thymus weight in group E was lower than that in group B, C and D. The tumor weight in group B and C was lower than that in group E. The tumor numbers among four groups were not statistically different. Blood T-cell proliferation rate in group C was higher than that in group E. Spleen T-cell proliferation index in group A was higher than that in group E and D. Compared with group E, serum SOD activities were increased and MDA significantly decreased in Tau groups. There were no significant differences in GSH-Px activities. The decrease of serum O^6-MeG was observed in Tau groups. Conclusion Taurine supplemented in diet can inhibit DMBA induced-tumor in rats. The antioxidation, immunomodulation, enhancement of DNA repair and inhibition of cell proliferation are the possible mechanisms. [ACTA NUTRIMENTA SINICA, 2008,30（1）： 57-60]

关 键 词：牛磺酸 二甲基苯蒽 大鼠 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]