The Expression and Bioinformatic Analysis of a Novel Gene C20orf14 Associated with Lymphoma  

作　　者：苏良平 陈登 章建民 李西明 潘桂红 白向阳 卢运萍 周剑峰 李双 

机构地区：[1]Department of Internal Medicine, Daye Iron Mine Hospital, Mining Industry, Wuhan Iron and Steel Group [2]Cancer Biology Center, Department of Obstetrics and Gynecology [3]Cancer Biology Center, Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2008年第1期97-101,共5页华中科技大学学报（医学英德文版）

基　　金：grants from National "973" Key Basic Research and Development Program Foundation (No. 2002CB513100) ;the transversal project of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (No. 2007004) of China

摘　　要：The aim of the present study was to explore the differentially expressed genes in the blood vessel endothelial cells （BVECs） between diffuse large B-cell lymphoma （DLBCL） and reactive lymph node hyperplasia （RLNH）, and to perform an initial bioinformatics analysis on a novel gene, C20orf14, which is highly expressed in lymph node of lymphoma. The mRNA of the tissue from the BVECs of DLBCL and RLNH tissues was labeled with biotin respectively and hybridized with expression profile microarray, and the differentially expressed genes were obtained. Initial bio-informatics analysis was performed on a novel gene named C20orf14. Its gene structure, genomic localization, the physical and chemical characteristics of the putative protein, subcellular localization, functional domain etc. were predicted, and the systematic evolution analysis was performed on the similar proteins among several species. By using expression profile microarray, many differentially expressed genes were uncovered. The efficient bioinformatics analysis have fundamentally identified that C20orf14 was a nuclear protein, and may be involved in the post-transcription modification of mRNA. Therefore, microarray is an efficient and high throughout strategy for the detection of differentially expressed genes, and C20orf14 is thought to be a potential target for tumor metastasis researches by bioinformatics analysis.The aim of the present study was to explore the differentially expressed genes in the blood vessel endothelial cells （BVECs） between diffuse large B-cell lymphoma （DLBCL） and reactive lymph node hyperplasia （RLNH）, and to perform an initial bioinformatics analysis on a novel gene, C20orf14, which is highly expressed in lymph node of lymphoma. The mRNA of the tissue from the BVECs of DLBCL and RLNH tissues was labeled with biotin respectively and hybridized with expression profile microarray, and the differentially expressed genes were obtained. Initial bio-informatics analysis was performed on a novel gene named C20orf14. Its gene structure, genomic localization, the physical and chemical characteristics of the putative protein, subcellular localization, functional domain etc. were predicted, and the systematic evolution analysis was performed on the similar proteins among several species. By using expression profile microarray, many differentially expressed genes were uncovered. The efficient bioinformatics analysis have fundamentally identified that C20orf14 was a nuclear protein, and may be involved in the post-transcription modification of mRNA. Therefore, microarray is an efficient and high throughout strategy for the detection of differentially expressed genes, and C20orf14 is thought to be a potential target for tumor metastasis researches by bioinformatics analysis.

关 键 词：MICROARRAY LYMPHOMA LCM BIOINFORMATICS C20orf14 

分 类 号：R733.1[医药卫生—肿瘤]